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Identification of mRNA markers for molecular staging of lymph nodes in colorectal cancer
- Source :
- Clinical chemistry. 52(3)
- Publication Year :
- 2006
-
Abstract
- Background: One evolving approach to improved prognostication of cancer patients is the identification of previously occult disease by use of quantitative reverse transcription-PCR. Surprisingly, no systematic analysis of potential mRNA markers for colorectal cancer has been reported. We therefore performed an extensive mRNA marker survey for colorectal cancers.Methods: We identified potential markers through literature and database searches. We analyzed all markers by quantitative reverse transcription-PCR on a limited set of primary tumors and benign lymph nodes. Selected markers were further evaluated on a larger tissue set with positive lymph nodes.Results: We evaluated 43 markers and undertook further analysis of 6 in the secondary screening. Five gene markers—CDX1, carcinoembryonic antigen (CEA), CK20, TACSTD1, and Villin1 (VIL1)—provided perfect classification of lymph node status.Conclusions: Several mRNA markers are capable of providing exceptionally accurate characterization of lymph node status in colorectal cancer. An automated, multimarker, quantitative reverse transcription-PCR assay for characterization of lymph nodes from colorectal cancer patients may be useful for improved staging and therapeutic decision making in colorectal cancer.
- Subjects :
- Oncology
medicine.medical_specialty
Pathology
Colorectal cancer
Clinical Biochemistry
chemistry.chemical_compound
Carcinoembryonic antigen
Molecular marker
Internal medicine
medicine
Biomarkers, Tumor
Humans
RNA, Messenger
Lymph node
Messenger RNA
biology
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Biochemistry (medical)
Cancer
medicine.disease
Prognosis
medicine.anatomical_structure
chemistry
Genetic marker
Lymphatic Metastasis
biology.protein
Lymph
Lymph Nodes
business
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 00099147
- Volume :
- 52
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Clinical chemistry
- Accession number :
- edsair.doi.dedup.....b855b0acf9608e7546549376440fb417