Back to Search
Start Over
Interaction of E1 and hSNF5 proteins stimulates replication of human papillomavirus DNA
- Source :
- Nature. 399:487-491
- Publication Year :
- 1999
- Publisher :
- Springer Science and Business Media LLC, 1999.
-
Abstract
- Mammalian viruses often use components of the host's cellular DNA replication machinery to carry out replication of their genomes, which enables these viruses to be used as tools for characterizing factors that are involved in cellular DNA replication. The human papillomavirus (HPV) E1 protein is essential for replication of the virus DNA1,2,3. Here we identify the cellular factor that participates in viral DNA replication by using a two-hybrid assay4 in the yeast Saccharomyces cerevisiae and E1 protein as bait. Using this assay, we isolated Ini1/hSNF5 (ref. 5), a component of the SWI/SNF complex which facilitates transcription by altering the structure of chromatin6. In vitro binding and immunoprecipitation confirmed that E1 interacts directly with Ini1/hSNF5. Transient DNA-replication assay revealed that HPV DNA replication is stimulated in a dose-dependent manner by addition of Ini1/hSNF5, and that Ini1/hSNF5 antisense RNA blocks the replication of HPV DNA. Amino-acid substitution at residues that are conserved among E1 proteins prevented the E1-Ini1/hSNF5 interaction and reduced DNA replication of HPV in vivo. Our results indicate that Ini1/hSNF5 is required for the efficient replication of papillomavirus DNA and is therefore needed, either alone or in complex with SWI/SNF complex, for mammalian DNA replication as well.
- Subjects :
- Saccharomyces cerevisiae Proteins
Chromosomal Proteins, Non-Histone
Recombinant Fusion Proteins
Eukaryotic DNA replication
Saccharomyces cerevisiae
Transfection
Virus Replication
Pre-replication complex
DNA replication factor CDT1
Viral Proteins
Replication factor C
Control of chromosome duplication
Minichromosome maintenance
Tumor Cells, Cultured
Humans
RNA, Antisense
Papillomaviridae
Binding Sites
Multidisciplinary
biology
DNA replication
Oncogene Proteins, Viral
SMARCB1 Protein
Molecular biology
Chromatin
DNA-Binding Proteins
DNA, Viral
Mutagenesis, Site-Directed
biology.protein
Origin recognition complex
HeLa Cells
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 14764687 and 00280836
- Volume :
- 399
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....b85195869d61108b8f59a68ddc79e508