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Intraperitoneal immune cell status in infertile women with and without endometriosis
- Source :
- Journal of Reproductive Immunology. 80:80-90
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Endometriosis is a widespread chronic disease characterized by endometrial tissue located outside the uterine cavity. Clinical signs are chronic pelvic pain and infertility. Emerging evidence indicates that the immune system is profoundly involved in the onset and/or progression of endometriosis. However, mechanistic pathways have not yet been conclusively specified. In this study, women undergoing diagnostic laparoscopy due to infertility were recruited, and classified as early-stage endometriosis (n=30), advanced-stage endometriosis (n=8) or no endometriosis (n=31). The frequency and phenotype of leukocytes were evaluated in peritoneal fluid. While the frequency of lymphocytes was not significantly different, neutrophils were increased in endometriosis. Flow cytometry analysis revealed an increased frequency of CD4(+) and CD8(+) cells in peritoneal fluid of endometriosis patients. In addition, the frequency of CD4(+)CD25(+)CD103(+) cells and lineage(-)HLA-DR(+)CD11c(+)CD123(+) dendritic cells was decreased in peritoneal fluid in endometriosis, whereas CD57(+) NK cells and CD8(+)CD28(-) T suppressor cells remained largely unaltered. We conclude that therapeutic approaches in endometriosis might focus on peritoneal leukocytes as a target or surveillance marker; however, immune alterations in peritoneal fluid are subtle and their analysis will require highly standardized and harmonized protocols.
- Subjects :
- Adult
Infertility
Pathology
medicine.medical_specialty
Immunology
Endometriosis
Cell Separation
Pelvic Pain
Natural killer cell
Immune system
Antigens, CD
Leukocytes
medicine
Ascitic Fluid
Humans
Immunology and Allergy
Cell Lineage
IL-2 receptor
Cell Proliferation
business.industry
Peritoneal fluid
Pelvic pain
Obstetrics and Gynecology
Cell Differentiation
Dendritic Cells
HLA-DR Antigens
Dendritic cell
Flow Cytometry
medicine.disease
medicine.anatomical_structure
Reproductive Medicine
Disease Progression
Female
Laparoscopy
medicine.symptom
business
Subjects
Details
- ISSN :
- 01650378
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Journal of Reproductive Immunology
- Accession number :
- edsair.doi.dedup.....b84c4dedb300458631b032894d3463cc