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Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
- Source :
- Journal of Insect Science
- Publication Year :
- 2020
- Publisher :
- Uppsala universitet, Mikrobiologi, 2020.
-
Abstract
- Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC50 and time to death after infection. The LC50 values of Nang Lai, NK04, and Sam Rong strains were 5.05–1.52 × 107 PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC50 values were 7.91–1.82 × 106 PIBs/ml and mortality peaked 4–5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU.
- Subjects :
- 0106 biological sciences
AcademicSubjects/SCI01382
Veterinary medicine
Severe disease
Virulence
01 natural sciences
Time to death
Microbiology
Virus
03 medical and health sciences
polyvoltine
Bombyx mori
Animals
030304 developmental biology
0303 health sciences
biology
Inoculation
Hexagonal crystal system
Research
fungi
General Medicine
biology.organism_classification
Bombyx
Thailand
Nucleopolyhedroviruses
virulence
010602 entomology
BmNPV-MSU
Mikrobiologi
Insect Science
Bombyx mori nucleopolyhedrovirus
Host-Pathogen Interactions
nucleopolyhedrovirus
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Insect Science
- Accession number :
- edsair.doi.dedup.....b840e2b72ec10c4d673415ae4780b0f8