Rapid high resolution melting assay to differentiate Streptococcus suis serotypes 2, 1/2, 1, and 14

Authors :: Fenja Rademacher
Roger Stephan
Patricia Landolt
Nathalie Spoerry Serrano
Simone Scherrer
Jacques Schrenzel
Marcelo Gottschalk
University of Zurich
Scherrer, Simone
Source :: MicrobiologyOpen, MicrobiologyOpen, Vol. 9, No 4 (2020), MicrobiologyOpen, Vol 9, Iss 4, Pp n/a-n/a (2020)
Publication Year :: 2020
Abstract: This rapid high resolution melting (HRM) assay allows distinguishing between Streptococcus suis serotype pairs 2 and 1/2 as well as 1 and 14, respectively, based on a single‐nucleotide polymorphism within capsular polysaccharide synthesis gene cluster K. This assay is easy to implement and identifies potential zoonotic serotypes.<br />The designed high resolution melting assay allows to correctly differentiate Streptococcus suis serotype pair 2 and 1/2 or 1 and 14, respectively. The assay is based on a single‐nucleotide polymorphism within the capsular polysaccharide synthesis gene cluster K and enables a rapid identification of potential zoonotic serotypes.

Language :: English
ISSN :: 20458827
Database :: OpenAIRE
Journal :: MicrobiologyOpen, MicrobiologyOpen, Vol. 9, No 4 (2020), MicrobiologyOpen, Vol 9, Iss 4, Pp n/a-n/a (2020)
Accession number :: edsair.doi.dedup.....b81fda61d8b6bb38b7387472eef75f4e
Full Text :: https://doi.org/10.5167/uzh-200128

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