Back to Search
Start Over
Theranostic, pH-Responsive, Doxorubicin-Loaded Nanoparticles Inducing Active Targeting and Apoptosis for Advanced Gastric Cancer
- Source :
- Biomacromolecules. 16(12)
- Publication Year :
- 2015
-
Abstract
- This study developed a kind of magnetic-polymer nanocarrier with folate receptor-targeting and pH-sensitive multifunctionalities to carry doxorubicin (DOX) for treatment of advanced gastric cancer (AGC). Folate-conjugated, pH-sensitive, amphiphilic poly(β-aminoester) self-assembled with hydrophobic oleic acid-modified iron oxide nanoparticles, and the resulting hydrophobic interaction area is a reservoir for lipophilic DOX (F-P-DOX). Confocal microscopy illustrated that F-P-DOX treatment could keep higher DOX accumulation in cells than P-DOX (without folate conjugation), and therefore get a higher efficiency of DOX internalization at pH 6.5 than at pH 7.4. Electron microscope characterization and real-time polymerase chain reaction revealed cell apoptosis promoted by F-P-DOX. The better efficacy of F-P-DOX on GC than free DOX and P-DOX was determined by MTT assay and xenograft model. Moreover, the accumulation of F-P-DOX in the tumor site was detected by magnetic resonance imaging (MRI). All those observations suggest F-P-DOX could be a promising theranostic candidate for AGC treatment.
- Subjects :
- Male
Polymers and Plastics
Apoptosis
02 engineering and technology
Ferric Compounds
Theranostic Nanomedicine
law.invention
chemistry.chemical_compound
Mice
0302 clinical medicine
law
polycyclic compounds
Materials Chemistry
Internalization
Magnetite Nanoparticles
media_common
Drug Carriers
Antibiotics, Antineoplastic
Chemistry
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Magnetic Resonance Imaging
Biochemistry
030220 oncology & carcinogenesis
Disease Progression
Magnets
0210 nano-technology
Drug carrier
Hydrophobic and Hydrophilic Interactions
Iron oxide nanoparticles
medicine.drug
media_common.quotation_subject
Mice, Nude
Bioengineering
macromolecular substances
Adenocarcinoma
Biomaterials
03 medical and health sciences
Folic Acid
Confocal microscopy
Stomach Neoplasms
Cell Line, Tumor
medicine
Animals
Humans
MTT assay
Doxorubicin
organic chemicals
technology, industry, and agriculture
Xenograft Model Antitumor Assays
carbohydrates (lipids)
Drug Liberation
Biophysics
Nanocarriers
Oleic Acid
Subjects
Details
- ISSN :
- 15264602
- Volume :
- 16
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules
- Accession number :
- edsair.doi.dedup.....b8040d180e2da747a0918d8b558e71ba