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Free and Nanoencapsulated tobramycin: effects on planktonic and biofilm forms of pseudomonas
- Source :
- Dipòsit Digital de la UB, Universidad de Barcelona, Microorganisms, Microorganisms; Volume 5; Issue 3; Pages: 35, Microorganisms, Vol 5, Iss 3, p 35 (2017)
- Publication Year :
- 2017
- Publisher :
- MDPI, 2017.
-
Abstract
- Cystic fibrosis (CF) is a genetic disorder in which frequent pulmonary infections develop secondarily. One of the major pulmonary pathogens colonizing the respiratory tract of CF patients and causing chronic airway infections is Pseudomonas aeruginosa. Although tobramycin was initially effective against P. aeruginosa, tobramycin-resistant strains have emerged. Among the strategies for overcoming resistance to tobramycin and other antibiotics is encapsulation of the drugs in nanoparticles. In this study, we explored the antimicrobial activity of nanoencapsulated tobramycin, both in solid lipid nanoparticles (SLN) and in nanostructured lipid carriers (NLC), against clinical isolates of P. aeruginosa obtained from CF patients. We also investigated the efficacy of these formulations in biofilm eradication. In both experiments, the activities of SLN and NLC were compared with that of free tobramycin. The susceptibility of planktonic bacteria was determined using the broth microdilution method and by plotting bacterial growth. The minimal biofilm eradication concentration (MBEC) was determined to assess the efficacy of the different tobramycin formulations against biofilms. The activity of tobramycin-loaded SLN was less than that of either tobramycin-loaded NLC or free tobramycin. The minimum inhibitory concentration (MIC) and MBEC of nanoencapsulated tobramycin were slightly lower (1-2 logs) than the corresponding values of the free drug when determined in tobramycin-susceptible isolates. However, in tobramycin-resistant strains, the MIC and MBEC did not differ between either encapsulated form and free tobramycin. Our results demonstrate the efficacy of nanoencapsulated formulations in killing susceptible P. aeruginosa from CF and from other patients. http://www.mdpi.com/2076-2607/5/3/35
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.drug_class
030106 microbiology
Antibiotics
tobramycin
lipid nanoparticles
antibacterial and antibiofilm effects
P. aeruginosa
cystic fibrosis
02 engineering and technology
Biology
medicine.disease_cause
Microbiology
Article
Cystic fibrosis
03 medical and health sciences
Minimum inhibitory concentration
Virology
medicine
Tobramycin
Medicaments antibacterians
lcsh:QH301-705.5
Nanopartícules
Pseudomonas aeruginosa
Broth microdilution
Pseudomonas
Biofilm
Fibrosi quística
021001 nanoscience & nanotechnology
Antimicrobial
biology.organism_classification
Lipids
carbohydrates (lipids)
Antibacterial agents
lcsh:Biology (General)
Lípids
Nanoparticles
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 20762607
- Database :
- OpenAIRE
- Journal :
- Dipòsit Digital de la UB, Universidad de Barcelona, Microorganisms, Microorganisms; Volume 5; Issue 3; Pages: 35, Microorganisms, Vol 5, Iss 3, p 35 (2017)
- Accession number :
- edsair.doi.dedup.....b7fecdaccb4749640e2acec9cef04804