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The inherent flexibility of type I non-ribosomal peptide synthetase multienzymes drives their catalytic activities
- Source :
- Open Biology, Open Biology, 2021, 11 (5), pp.200386. ⟨10.1098/rsob.200386⟩, Open Biology, Royal Society, 2021, 11 (5), pp.200386. ⟨10.1098/rsob.200386⟩, Open Biology, Vol 11, Iss 5 (2021)
- Publication Year :
- 2021
-
Abstract
- International audience; Non-ribosomal peptide synthetases (NRPSs) are multienzymes that produce complex natural metabolites with many applications in medicine and agriculture. They are composed of numerous catalytic domains that elongate and chemically modify amino acid substrates or derivatives and of non-catalytic carrier protein domains that can tether and shuttle the growing products to the different catalytic domains. The intrinsic flexibility of NRPSs permits conformational rearrangements that are required to allow interactions between catalytic and carrier protein domains. Their large size coupled to this flexibility renders these multi-domain proteins very challenging for structural characterization. Here, we summarize recent studies that offer structural views of multi-domain NRPSs in various catalytically relevant conformations, thus providing an increased comprehension of their catalytic cycle. A better structural understanding of these multienzymes provides novel perspectives for their re-engineering to synthesize new bioactive metabolites.
- Subjects :
- QH301-705.5
Immunology
Peptide Synthetases
Peptide
Computational biology
Review
Biology
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Catalysis
03 medical and health sciences
Structure-Activity Relationship
Catalytic Domain
supramodular architecture
Biology (General)
Peptide Synthases
Review Articles
030304 developmental biology
chemistry.chemical_classification
Flexibility (engineering)
0303 health sciences
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]
non-ribosomal peptide synthetases
010405 organic chemistry
General Neuroscience
Ribosomal RNA
0104 chemical sciences
3. Good health
Amino acid
flexibility
chemistry
Catalytic cycle
Carrier protein
Subjects
Details
- ISSN :
- 20462441
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Open biology
- Accession number :
- edsair.doi.dedup.....b7e8d9082b6c8808f757f90c6a6bee4e