Back to Search
Start Over
Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in children in Egypt
Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in children in Egypt
- Source :
- Parasites & Vectors, Parasites & Vectors, Vol 11, Iss 1, Pp 1-9 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background The transmission of Cryptosporidium spp. and Giardia duodenalis into humans varies according to species/genotypes of the pathogens. Although infections with both parasites are recorded in Egypt, few data are available on the distribution of Cryptosporidium species and G. duodenalis genotypes. The present study assessed the occurrence and genetic diversity of Cryptosporidium spp. and G. duodenalis in Egyptian children. Methods In the present study, 585 fecal specimens were collected from children eight years old and younger in three provinces (El-Dakahlia, El-Gharbia and Damietta) during March 2015 to April 2016. PCR-RFLP analysis of the small subunit rRNA gene and sequence analysis of the 60 kDa glycoprotein gene were used to detect and subtype Cryptosporidium spp., respectively, whereas PCR and sequence analyses of the triose phosphate isomerase, glutamate dehydrogenase and β-giardin genes were used to detect and genotype Giardia duodenalis. Results The overall infection rates of Cryptosporidium spp. and G. duodenalis were 1.4% and 11.3%, respectively. The Cryptosporidium species identified included C. hominis and C. parvum, each with three subtype families. The C. hominis subtypes were IbA6G3 (n = 2), IdA17 (n = 1), IdA24 (n = 1) and IfA14G1R5 (n = 1), while C. parvum subtypes were IIdA20G1 (n = 1), IIaA15G2R1 (n = 1), and IIcA5G3a (n = 1). The G. duodenalis identified included both assemblages A (n = 31) and B (n = 34). All G. duodenalis assemblage A belonged to the anthroponotic sub-assemblage AII, while a high genetic heterogeneity was seen within assemblage B. Conclusions Data from this study are useful in our understanding of the genetic diversity of Cryptosporidium spp. and G. duodenalis in Egypt and the potential importance of anthroponotic transmission in the epidemiology of both pathogens. Electronic supplementary material The online version of this article (10.1186/s13071-018-2981-7) contains supplementary material, which is available to authorized users.
- Subjects :
- Giardiasis
Male
0301 basic medicine
Genotype
Epidemiology
030231 tropical medicine
Protozoan Proteins
Cryptosporidiosis
Cryptosporidium
medicine.disease_cause
Polymerase Chain Reaction
lcsh:Infectious and parasitic diseases
Microbiology
law.invention
Feces
03 medical and health sciences
0302 clinical medicine
law
parasitic diseases
Genetic variation
medicine
Animals
Humans
Giardia lamblia
lcsh:RC109-216
Child
Children
Phylogeny
Polymerase chain reaction
Subtypes
Genetic diversity
biology
Research
Genetic Variation
Infant
DNA, Protozoan
030108 mycology & parasitology
Ribosomal RNA
biology.organism_classification
Infectious Diseases
Parasitology
Child, Preschool
Egypt
Female
Giardia duodenalis
Subjects
Details
- ISSN :
- 17563305
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Parasites & Vectors
- Accession number :
- edsair.doi.dedup.....b7e7448e45287a596dc196a72d9319c0
- Full Text :
- https://doi.org/10.1186/s13071-018-2981-7