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Carbonic anhydrase activity in tissues of the icefish Chionodraco hamatus and of the red-blooded teleosts Trematomus bernacchii and Anguilla anguilla
- Source :
- Journal of Experimental Biology. 204:3983-3992
- Publication Year :
- 2001
- Publisher :
- The Company of Biologists, 2001.
-
Abstract
- SUMMARY Carbonic anhydrase (CA) activity was measured in blood, intestine, kidney and gill of two Antarctic teleosts, the haemoglobinless Chionodraco hamatus and the red-blooded Trematomus bernacchii, and of the temperate teleost Anguilla anguilla. In all species, the highest CA activity was in the gills, with the greatest activity in C. hamatus. CA activity in the blood was highest in A. anguilla, but none was detected in the blood of C. hamatus despite the presence of plasma CA inhibitors. The enzyme was present but its activity was low in the intestine and kidney of all three species. The existence of very high CA activity in C. hamatus gills compared with the red-blooded species was investigated further by isolating and characterising the branchial cytosolic CA isoforms. The turnover rate of the C. hamatus isoform was significantly higher than that of T. bernacchii and A. anguilla. The isoforms from both the Antarctic species exhibited lower apparent Km (Km,app) and heat stability than those from A. anguilla. Sensitivity to sulphonamides was similar in all species and was within the range of the mammalian CA II isoform. The branchial CA isoforms of C. hamatus, T. bernacchii and A. anguilla displayed relative molecular masses of 28.9, 29.9 and 31.2 kDa, respectively. The results suggest that the hemoglobinless teleost possesses a different branchial cytosolic CA isoform from that of red-blooded teleosts.
- Subjects :
- Gills
Gill
Gene isoform
animal structures
Nothotheniodei
Physiology
carbonic anhydrase
Antarctic Regions
Antarctic teleost
Trematomus bernacchii
Aquatic Science
Kidney
Hemoglobins
blood
Chionodraco hamatus
Carbonic anhydrase
Trematomus
medicine
Animals
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Carbonic Anhydrases
Chaenichthyidae
chemistry.chemical_classification
Anguilla anguilla
biology
Fishes
Carbonic anhydrase activity
gill
Anatomy
Anguilla
biology.organism_classification
pH homeostasi
Intestines
medicine.anatomical_structure
Enzyme
Biochemistry
chemistry
haemoglobinle
Chionodraco hamatu
Insect Science
biology.protein
Animal Science and Zoology
Subjects
Details
- ISSN :
- 14779145 and 00220949
- Volume :
- 204
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Biology
- Accession number :
- edsair.doi.dedup.....b7e4f6cbd7ef0efa59e694d12876964e