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Single‐cell transcriptomes of murine bone marrow stromal cells reveal niche‐associated heterogeneity
- Source :
- European Journal of Immunology
- Publication Year :
- 2019
- Publisher :
- John Wiley and Sons Inc., 2019.
-
Abstract
- Bone marrow (BM) stromal cells are important in the development and maintenance of cells of the immune system. Using single cell RNA sequencing, we here explore the functional and phenotypic heterogeneity of individual transcriptomes of 1167 murine BM mesenchymal stromal cells. These cells exhibit a tremendous heterogeneity of gene expression, which precludes the identification of defined subpopulations. However, according to the expression of 108 genes involved in the communication of stromal cells with hematopoietic cells, we have identified 14 non‐overlapping subpopulations, with distinct cytokine or chemokine gene expression signatures. With respect to the maintenance of subsets of immune memory cells by stromal cells, we identified distinct subpopulations expressing Il7, Il15 and Tnfsf13b. Together, this study provides a comprehensive dissection of the BM stromal heterogeneity at the single cell transcriptome level and provides a basis to understand their lifestyle and their role as organizers of niches for the long‐term maintenance of immune cells.
- Subjects :
- Stromal cell
bone marrow
medicine.medical_treatment
Short Communication
Immunology
Bone Marrow Cells
Biology
single cell sequencing
Transcriptome
Mice
Immune system
hematopoietic cells
B-Cell Activating Factor
medicine
Immunology and Allergy
Animals
Basic
Cells, Cultured
Interleukin-15
Sequence Analysis, RNA
Interleukin-7
Mesenchymal stem cell
Mesenchymal Stem Cells
Hematopoietic Stem Cells
cytokines
Cell biology
Short Communication|Basic
Mice, Inbred C57BL
Haematopoiesis
Cytokine
medicine.anatomical_structure
Single cell sequencing
Systems immunology
Bone marrow
Stromal Cells
Subjects
Details
- Language :
- English
- ISSN :
- 15214141 and 00142980
- Volume :
- 49
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....b7df5984d6c31b1948591d7b7764aa3d