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Domain- and Site-Specific Phosphorylation of Bovine NF-L by Rho-Associated Kinase
Domain- and Site-Specific Phosphorylation of Bovine NF-L by Rho-Associated Kinase
- Source :
- Biochemical and Biophysical Research Communications. 245:407-411
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Rho-associated kinase (Rho-kinase), the putative target of the small GTP-binding protein Rho, phosphorylated neurofilament protein (NF-L) in vitro with approximately 1 mole phosphate per mole NF-L. Phosphorylated NF-L no longer formed the 10 nm filaments, and NF-L filaments were phosphorylated with a result of nearly complete disassembly. NF-L phosphorylated by Rho-kinase was digested with trypsin, and digested fragments were assigned by MALDI/TOF. Unique phosphorylation sites were found at Ser-26 and Ser-57 in the head domain of NF-L. These results indicate that domain- and site-specific phosphorylation by Rho-kinase may regulate the assembly-disassembly of NF-L filaments.
- Subjects :
- inorganic chemicals
Neurofilament
Molecular Sequence Data
Biophysics
Nerve Tissue Proteins
macromolecular substances
Protein Serine-Threonine Kinases
environment and public health
Biochemistry
Phosphates
chemistry.chemical_compound
Neurofilament Proteins
Mole
medicine
Animals
Trypsin
Protein phosphorylation
Amino Acid Sequence
Phosphorylation
Molecular Biology
rho-Associated Kinases
Chemistry
Kinase
Intracellular Signaling Peptides and Proteins
Cell Biology
Phosphate
Molecular biology
Peptide Fragments
Kinetics
Microscopy, Electron
enzymes and coenzymes (carbohydrates)
Guanosine 5'-O-(3-Thiotriphosphate)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Domain (ring theory)
bacteria
Cattle
Sequence Alignment
Sequence Analysis
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 245
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....b7c471e84b9751a1b72fd0402c515a48
- Full Text :
- https://doi.org/10.1006/bbrc.1998.8446