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Detection of cryptic chromosomal lesions including acquired segmental uniparental disomy in advanced and low-risk myelodysplastic syndromes
- Source :
- Experimental Hematology. 35:1728-1738
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Objectives Using metaphase cytogenetics (MC), chromosomal defects can be detected in 40% to 60% of patients with myelodysplastic syndromes (MDS); cytogenetic results have a major impact on prognosis. We hypothesize that more precise methods of chromosomal analysis will detect new/additional cryptic lesions in a higher proportion of MDS patients. Methods We have applied single nucleotide polymorphism microarrays (SNP-A) to perform high-resolution karyotyping in MDS to determine gene copy number and detect loss of heterozygosity (LOH). Results Using this method, chromosomal defects were found in 82% of MDS patients vs 50% as measured by MC; lesions were present in 68% of patients with normal MC, while in 81% of those with abnormal MC, new aberrations were found. In addition to gains or losses of chromosomal material, areas of LOH due to segmental uniparental disomy were found in 33% of patients. Conclusion SNP-A findings demonstrate that chromosomal lesions are present in a much higher proportion of patients than predicted by traditional cytogenetics. These lesions may reflect an underlying generalized chromosomal instability in MDS. Additional previously cryptic defects may explain the clinical variability of MDS. New lesions may have important prognostic implications, suggesting that, in the future, SNP-A–based karyotyping may complement MC in laboratory evaluation of MDS.
- Subjects :
- Adult
Cancer Research
Pathology
medicine.medical_specialty
Gene Dosage
Loss of Heterozygosity
Single-nucleotide polymorphism
Biology
Polymorphism, Single Nucleotide
Loss of heterozygosity
hemic and lymphatic diseases
Chromosome instability
Genetics
medicine
Humans
Copy-number variation
Molecular Biology
Aged
Oligonucleotide Array Sequence Analysis
Aged, 80 and over
Chromosome Aberrations
Myelodysplastic syndromes
Cytogenetics
Karyotype
Cell Biology
Hematology
Middle Aged
Uniparental Disomy
medicine.disease
Uniparental disomy
Case-Control Studies
Karyotyping
Myelodysplastic Syndromes
Subjects
Details
- ISSN :
- 0301472X
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Experimental Hematology
- Accession number :
- edsair.doi.dedup.....b79fc6e308ea14e34dd1ab4557d9ce30