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Adverse effects of MVA-T7 on the transport of Marburg virus glycoprotein
- Source :
- Journal of virological methods. 91(1)
- Publication Year :
- 2001
-
Abstract
- Expression of glycoproteins has been carried out successfully using recombinant vaccinia virus vectors. Especially attractive is the use of recombinant vaccinia viruses which express the DNA-dependent RNA polymerase of the phage T7 (T7-polymerase). The T7-polynerase drives the transcription of plasmid-based genes under the control of the T7 RNA polymerase promoter transfected into the infected cell. Comparison of two different recombinant vaccinia viruses, vTF7-3 and MVA-T7, revealed that post-translational processing of Marburg virus surface glycoprotein (GP) is impaired in the MVA-T7 but not in the vTF7-3 system. Influenza virus hemagglutinin, however, was transported and processed like the authentic protein in both systems. It is shown that transport of GP in the MVA-T7 system is not completely blocked, but the vast majority of molecules remained Endo H-sensitive. Only trace amounts evaded the endoplasmatic reticulum and reached the plasma membrane. Thus, the adverse effects of MVA-T7 on the processing of recombinant glycoproteins cannot be predicted, and correct processing has to be investigated for every expressed glycoprotein.
- Subjects :
- viruses
Genetic Vectors
Hemagglutinins, Viral
Vaccinia virus
Biology
Recombinant virus
Endoplasmic Reticulum
Virus
law.invention
Marburg virus
chemistry.chemical_compound
Plasmid
Viral Envelope Proteins
law
Virology
RNA polymerase
medicine
T7 RNA polymerase
Humans
chemistry.chemical_classification
Cell Membrane
Molecular biology
Protein Transport
chemistry
Influenza A virus
Recombinant DNA
Glycoprotein
Protein Processing, Post-Translational
medicine.drug
HeLa Cells
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 91
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....b786bfc1064f71164eeba0e19693884a