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Identification and characterization of Hoxa9 binding sites in hematopoietic cells
- Source :
- Blood. 119:388-398
- Publication Year :
- 2012
- Publisher :
- American Society of Hematology, 2012.
-
Abstract
- The clustered homeobox proteins play crucial roles in development, hematopoiesis, and leukemia, yet the targets they regulate and their mechanisms of action are poorly understood. Here, we identified the binding sites for Hoxa9 and the Hox cofactor Meis1 on a genome-wide level and profiled their associated epigenetic modifications and transcriptional targets. Hoxa9 and the Hox cofactor Meis1 cobind at hundreds of highly evolutionarily conserved sites, most of which are distant from transcription start sites. These sites show high levels of histone H3K4 monomethylation and CBP/P300 binding characteristic of enhancers. Furthermore, a subset of these sites shows enhancer activity in transient transfection assays. Many Hoxa9 and Meis1 binding sites are also bound by PU.1 and other lineage-restricted transcription factors previously implicated in establishment of myeloid enhancers. Conditional Hoxa9 activation is associated with CBP/P300 recruitment, histone acetylation, and transcriptional activation of a network of proto-oncogenes, including Erg, Flt3, Lmo2, Myb, and Sox4. Collectively, this work suggests that Hoxa9 regulates transcription by interacting with enhancers of genes important for hematopoiesis and leukemia.
- Subjects :
- Epigenomics
Chromatin Immunoprecipitation
Hematopoiesis and Stem Cells
Blotting, Western
Immunology
Bone Marrow Cells
Enhancer RNAs
Biology
Real-Time Polymerase Chain Reaction
Biochemistry
Mice
Biomarkers, Tumor
Animals
MYB
RNA, Messenger
Myeloid Ecotropic Viral Integration Site 1 Protein
Hox gene
Enhancer
Transcription factor
Oligonucleotide Array Sequence Analysis
Homeodomain Proteins
Genetics
Binding Sites
Leukemia
Gene Expression Regulation, Leukemic
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Acetylation
Cell Biology
Hematology
Hematopoiesis
Neoplasm Proteins
Mice, Inbred C57BL
Enhancer Elements, Genetic
Histone
biology.protein
Female
Chromatin immunoprecipitation
Transcription Factors
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....b782af59f45a92120a603da65c7f6b3d