FSH and Its Second Messenger cAMP Stimulate the Transcription of Human Anti-Müllerian Hormone in Cultured Granulosa Cells

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance, a member of the TGF-β family, is responsible for the regression of Müllerian ducts in the male fetus. In females, AMH is synthesized by granulosa cells of preantral and small antral follicles, and production wanes at later stages of follicle maturation. Using RT-PCR in luteal granulosa cells in primary culture and reporter gene techniques in the KK1 granulosa cell line, we show that FSH and cAMP enhance AMH transcription, and LH has an additive effect. Gonadotropins and cAMP act through protein kinase A and p38 MAPK signaling pathways and involve the GATA binding factor-4 and steroidogenic factor-1 transcription factors, among others. The expression profile ofAMHand the dynamics of serumAMHafter gonadotropin stimulation have been interpreted as a down-regulating effect of FSH upon AMH production by granulosa cells. The specific effect of gonadotropins upon granulosa cells may be obscured in vivo by the effect of FSH upon follicular maturation and by the presence of other hormones and growth factors, acting individually or in concert. Fil: Taieb, Joëlle. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Grynberg, Michaël. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Pierre, Alice. Hôpital Antoine Béclère; Francia. Université Paris Sud; Francia Fil: Arouche, Nassim. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Massart, Perrine. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Belville, Corinne. Hôpital Antoine Béclère; Francia. Université Paris Sud; Francia Fil: Hesters, Laetitia. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Frydman, René. Hôpital Antoine Béclère; Francia. Université Paris Sud; Francia Fil: Catteau Jonard, Sophie. Universidad de Lille; Francia. Jeanne de Flandre Hospital; Francia Fil: Fanchin, Renato. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Picard, Jean-Yves. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Josso, Nathalie. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia Fil: Rey, Rodolfo Alberto. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: di Clemente, Nathalie. Université Paris Sud; Francia. Hôpital Antoine Béclère; Francia