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Acacetin inhibits invasion, migration and TGF-β1-induced EMT of gastric cancer cells through the PI3K/Akt/Snail pathway
- Source :
- BMC Complementary Medicine and Therapies, Vol 22, Iss 1, Pp 1-12 (2022), BMC Complementary Medicine and Therapies
- Publication Year :
- 2022
- Publisher :
- BMC, 2022.
-
Abstract
- Background Epithelial-to-mesenchymal transition (EMT) is a pivotal cellular phenomenon involved in tumour metastasis and progression. In gastric cancer (GC), EMT is the main reason for recurrence and metastasis in postoperative patients. Acacetin exhibits various biological activities. However, the inhibitory effect of acacetin on EMT in GC is still unknown. Herein, we explored the possible mechanism of acacetin on EMT in GC in vitro and in vivo. Methods In vitro, MKN45 and MGC803 cells were treated with acacetin, after which cell viability was detected by CCK-8 assays, cell migration and invasion were detected by using Transwell and wound healing assays, and protein expression was analysed by western blots and immunofluorescence staining. In vivo, a peritoneal metastasis model of MKN45 GC cells was used to investigate the effects of acacetin. Results Acacetin inhibited the proliferation, invasion and migration of MKN45 and MGC803 human GC cells by regulating the expression of EMT-related proteins. In TGF-β1-induced EMT models, acacetin reversed the morphological changes from epithelial to mesenchymal cells, and invasion and migration were limited by regulating EMT. In addition, acacetin suppressed the activation of PI3K/Akt signalling and decreased the phosphorylation levels of TGF-β1-treated GC cells. The in vivo experiments demonstrated that acacetin delayed the development of peritoneal metastasis of GC in nude mice. Liver metastasis was restricted by altering the expression of EMT-related proteins. Conclusion Our study showed that the invasion, metastasis and TGF-β1-induced EMT of GC are inhibited by acacetin, and the mechanism may involve the suppression of the PI3K/Akt/Snail signalling pathway. Therefore, acacetin is a potential therapeutic reagent for the treatment of GC patients with recurrence and metastasis.
- Subjects :
- Male
Mice, Inbred BALB C
Epithelial-Mesenchymal Transition
Research
Epithelial-to-mesenchymal transition (EMT)
Flavones
Acacetin
Metastasis
Transforming Growth Factor beta1
Disease Models, Animal
Mice
Phosphatidylinositol 3-Kinases
Other systems of medicine
Complementary and alternative medicine
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
TGF-β1
Animals
Humans
Snail Family Transcription Factors
Gastric cancer (GC)
Proto-Oncogene Proteins c-akt
RZ201-999
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 22
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Complementary Medicine and Therapies
- Accession number :
- edsair.doi.dedup.....b77744729d89ae5321f449884443150b