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Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles
- Source :
- Journal of Nanomaterials, Vol 2019 (2019)
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- This study was performed to investigate the biocompatibility (BC), magnetothermal effect, and DNA binding biological characteristics of manganese zinc ferrite nanoparticles (Mn0.6Zn0.4Fe2O4-NPs (MZF-NPs)) coated with pegylated manganese (PEG-MZF-NPs). Their functions as gene transfer carrier for gene therapy and magnetic medium for tumor hyperthermia were also explored. The manganese zinc ferrite nanoparticles were synthesized through high temperature cracking, and their characterizations were discovered. Hemolysis test and MTT assay were performed to evaluate biocompatibility, and their self-heating effects in the alternating magnetic field were investigated. PEG-MZF-NPs with different concentrations were measured by using 7.0 T Micro-MR scanner (MRI) to calculate the T2 value and r2 relaxation rate of each sample. The CD44-shRNA plasmids were constructed, and their ability to bind PEG-MZF-NPs were examined. The DNA release from PEG-MZF-NP/DNA complex and protection of DNA from nuclease digestion were also detected. After CD44-shRNA-EGFP were transfected into the ovarian cancer SK-OV-3 cells by using PEG-MZF-NPs as carriers, the transfection efficiency was detected by a flow cytometer and expression of CD44 mRNA and protein in cells was detected using RT-PCR and Western blot, respectively. We successfully prepared PEG-MZF-NPs with favorable dispersity, magnetic responsiveness, and BC. Typically, the excellent magnetothermal effect can be used for a tumor magnetothermal therapeutic study. In vitro MRI showed the application potential for being magnetic resonance T2 relaxation contrast agents and the possibility to achieve goal of integration of targeting diagnosis and treatment. The CD44-shRNA plasmids have been successfully constructed and concluded that PEG-MZF-NPs may serve as gene transfer carriers for gene therapy.
- Subjects :
- Materials science
Article Subject
Biocompatibility
technology, industry, and agriculture
chemistry.chemical_element
Nanoparticle
02 engineering and technology
Transfection
Manganese
021001 nanoscience & nanotechnology
In vitro
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Plasmid
chemistry
030220 oncology & carcinogenesis
lcsh:Technology (General)
Biophysics
lcsh:T1-995
General Materials Science
MTT assay
0210 nano-technology
DNA
Subjects
Details
- ISSN :
- 16874129 and 16874110
- Volume :
- 2019
- Database :
- OpenAIRE
- Journal :
- Journal of Nanomaterials
- Accession number :
- edsair.doi.dedup.....b76ea0b1201caea5225f7e644240e43f