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Functional motor neurons differentiating from mouse multipotent spinal cord precursor cells in culture and after transplantation into transected sciatic nerve
- Source :
- Journal of Neurosurgery. 98:1094-1103
- Publication Year :
- 2003
- Publisher :
- Journal of Neurosurgery Publishing Group (JNSPG), 2003.
-
Abstract
- Object. One of the current challenges in neurobiology is to ensure that neural precursor cells differentiate into specific neuron types, so that they can be used for transplantation purposes in patients with neuron loss. The goal of this study was to determine if spinal cord precursor cells could differentiate into motor neurons both in culture and following transplantation into a transected sciatic nerve. Methods. In cultures with trophic factors, neurons differentiate from embryonic precursor cells and express motor neuronal markers such as choline acetyltransferase (ChAT), Islet-1, and REG2. Reverse transcription—polymerase chain reaction analysis has also demonstrated the expression of Islet-1 in differentiated cultures. A coculture preparation of neurospheres and skeletal myocytes was used to show the formation of neuromuscular connections between precursor cell—derived neurons and myocytes both immunohistochemically and electrophysiologically. Following various survival intervals, precursor cells transplanted distal to a transection of the sciatic nerve differentiated into neurons expressing the motor neuron markers ChAT and the α11.2 (class C, L-type) voltage-sensitive Ca++ channel subunit. These cells extended axons into the muscle, where they formed cholinergic terminals. Conclusions. These results demonstrate that motor neurons can differentiate from spinal cord neural precursor cells grown in culture as well as following transplantation into a transected peripheral nerve.
- Subjects :
- Cell Survival
LIM-Homeodomain Proteins
Nerve Tissue Proteins
Biology
Choline O-Acetyltransferase
Mice
Fetal Tissue Transplantation
Neurosphere
Precursor cell
medicine
Animals
Muscle, Skeletal
Ultrasonography
Homeodomain Proteins
Motor Neurons
Mice, Inbred BALB C
Reverse Transcriptase Polymerase Chain Reaction
Multipotent Stem Cells
Cell Differentiation
Motor neuron
Spinal cord
Sciatic Nerve
Choline acetyltransferase
Axons
Nerve Regeneration
Transplantation
medicine.anatomical_structure
Microscopy, Fluorescence
Spinal Cord
nervous system
Synapses
Sciatic nerve
Stem cell
Neuroscience
Transcription Factors
Subjects
Details
- ISSN :
- 00223085
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Journal of Neurosurgery
- Accession number :
- edsair.doi.dedup.....b71a5fa9def8f451a21a9d429044aefe