Back to Search
Start Over
Genetic Relatedness ofEscherichia coliO157 Isolates from Cattle Feces and Preintervention Beef Carcasses
- Source :
- Foodborne Pathogens and Disease. 7:357-365
- Publication Year :
- 2010
- Publisher :
- Mary Ann Liebert Inc, 2010.
-
Abstract
- Our objective was to define and compare pulsed-field gel electrophoresis (PFGE) profiles of Escherichia coli O157 isolated from cattle feces and carcass samples to evaluate relationships between beef carcass contamination and fecal shedding of E. coli O157 at harvest. We used PFGE separation of Xba1-digested DNA to characterize E. coli O157 isolates (n = 174) from preevisceration carcasses (n = 39) and feces (n = 135) that were recovered from 37 E. coli O157-positive truckloads sampled at a commercial abattoir. Semiquantitative fecal culture techniques differentiated high-shedding, low-shedding, and negative cattle. Among all isolates, there were 17 PFGE types (95% homology) and 37 subtypes (100% homology). Specific subtypes were detected on multiple occasions and from different sample types within loads, among loads, and among days. Seventeen subtypes were recovered from carcasses; most were also recovered from feces of high-shedding cattle (13) and low-shedding cattle (14). Within truckload, the percentages of carcass isolates that were identical to high-shedder or low-shedder fecal isolates, as determined by PFGE, were 69.2% and 46.0%, respectively, whereas among different truckloads within the same study day, the percentages of carcass isolates that were the same subtype as high-shedder or low-shedder fecal isolates were 35.3% and 58.8%, respectively. Our results suggest that cattle feces from both low- and high-shedders pose a potential risk for E. coli O157 contamination of carcasses. Truckload may be an important factor in the potential transmission of E. coli O157, but isolates from carcasses also may be similar to those from feces of cattle on different truckloads and harvest days.
- Subjects :
- Meat
Time Factors
Food Handling
Colony Count, Microbial
Biology
Escherichia coli O157
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
Feces
fluids and secretions
Animal science
Genotype
Pulsed-field gel electrophoresis
medicine
Animals
Food microbiology
Animal Husbandry
Escherichia coli
Phylogeny
Bacterial Shedding
Rectum
DNA Fingerprinting
Bacterial Typing Techniques
Electrophoresis, Gel, Pulsed-Field
DNA profiling
Food Microbiology
Cattle
Animal Science and Zoology
Genetic relatedness
Food Science
Food contaminant
Subjects
Details
- ISSN :
- 15567125 and 15353141
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Foodborne Pathogens and Disease
- Accession number :
- edsair.doi.dedup.....b70d03f525cbaa1c6818fd929283da85