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Increased O2·−Production and Upregulation of ETBReceptors by Sympathetic Neurons in DOCA-Salt Hypertensive Rats
- Source :
- Hypertension. 43:1048-1054
- Publication Year :
- 2004
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2004.
-
Abstract
- Superoxide anion (O2·−) production is elevated in the vasculature of hypertensive animals but it is not known if O2·−production is also elevated in the sympathetic nervous system. We measured O2·−levels in prevertebral sympathetic ganglia of deoxycorticosterone acetate (DOCA)-salt hypertensive rats using the dihydroethidine (DHE) fluorescence method. O2·−was elevated in ganglia from DOCA-salt rats compared with normotensive sham rats. Treatment of ganglia with endothelin (ET)-1 (3×10−8mol/L) resulted in a 200% increase in fluorescence intensity in neurons, which was attenuated by the ETBreceptor antagonist BQ788 (10−7mol/L). ET-1 also increased the O2·−induced fluorescence in dissociated sympathetic neurons and PC-12 cells via activation of ETBreceptors, but not ETAreceptors. To evaluate whether elevated ET-1 levels in the ganglia might contribute to the elevated O2·−found in ganglia we measured the amount of ET-1 using an ELISA assay. ET-1 levels in sham rat celiac ganglia were 695.6±40.9 picogram per gram; they were not different than ET-1 levels in ganglia from DOCA-salt rats. We then compared ETBreceptor levels in ganglia from sham and DOCA-salt animals. ETBreceptor mRNA levels were 32% higher and ETBreceptor protein levels were 20% higher in celiac ganglia from DOCA-salt rats than from sham rats separately. In conclusion, O2·−is elevated in prevertebral sympathetic ganglia in DOCA-salt hypertension, and ET-1 is a potent stimulus for the elevation of O2·−levels in sympathetic ganglia, an effect that may be mediated by the upregulation of ETBreceptors.
- Subjects :
- Male
medicine.medical_specialty
Sympathetic nervous system
Endothelin A Receptor Antagonists
medicine.drug_class
Viper Venoms
PC12 Cells
Rats, Sprague-Dawley
chemistry.chemical_compound
Sympathetic Fibers, Postganglionic
Piperidines
Downregulation and upregulation
Superoxides
Internal medicine
Nerve Growth Factor
Internal Medicine
Animals
Medicine
Sodium Chloride, Dietary
Desoxycorticosterone
Receptor
education
education.field_of_study
Ganglia, Sympathetic
Endothelin-1
business.industry
Superoxide
Receptor antagonist
Receptor, Endothelin B
Endothelin 1
Rats
Up-Regulation
Endothelin 3
Oxidative Stress
medicine.anatomical_structure
Endocrinology
chemistry
Hypertension
Reactive Oxygen Species
business
Endothelin receptor
Oligopeptides
Subjects
Details
- ISSN :
- 15244563 and 0194911X
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Hypertension
- Accession number :
- edsair.doi.dedup.....b70242c660d63e4561bc26a553e14c1c