Back to Search
Start Over
Different reactivities of enzyme-linked immunosorbent assays for hepatocyte growth factor
- Source :
- Clinica Chimica Acta. 402:42-46
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Background Hepatocyte growth factor (HGF) plays diverse roles in organ development, tissue regeneration, and tumor progression. Measurement of HGF concentrations in blood and tissues using enzyme-linked immunosorbent assay (ELISA) is a simple and easy way to understand the significance of HGF in tissue regeneration, pathophysiology, and diagnosis. Methods We evaluated 3 ELISA kits from different sources, referred to herein as kits A, B, and C for convenience. Results We found that the concentrations of human HGF determined using ELISA vary significantly depending on the source of the ELISA kit. Kits A and B detected both single-chain pro-HGF and 2-chain mature HGF, but kit C detected only 2-chain HGF. A difference in reactivity was also detected during analysis of plasma samples. When rat plasma collected 4 h after subcutaneous administration of human HGF was analyzed, the HGF concentration determined using kit B was remarkably higher than those obtained using kits A and C. Results of a biological assay and Western blot analysis indicated that kit B detects even degraded HGF, by which the HGF concentrations determined using kit B were significantly overestimated. Conclusions This information serves as a guide for the selection of ELISA kits for human HGF.
- Subjects :
- Male
Clinical Biochemistry
Enzyme-Linked Immunosorbent Assay
Biology
Sensitivity and Specificity
Biochemistry
Cell Line
law.invention
Rats, Sprague-Dawley
Dogs
Western blot
law
medicine
Animals
Humans
Bioassay
Pancreatic elastase
chemistry.chemical_classification
Pancreatic Elastase
medicine.diagnostic_test
Hepatocyte Growth Factor
Biochemistry (medical)
General Medicine
Molecular biology
Recombinant Proteins
Rats
Enzyme
chemistry
Cell culture
Tumor progression
Recombinant DNA
Hepatocyte growth factor
Reagent Kits, Diagnostic
medicine.drug
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 402
- Database :
- OpenAIRE
- Journal :
- Clinica Chimica Acta
- Accession number :
- edsair.doi.dedup.....b700ecf6f15337ab4d8683b1dc80374a