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Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis

Authors :
C. Melissa Dobson
Fred D. Mast
Richard A. Rachubinski
Ramsey A. Saleem
Jennifer J. Smith
Rose Long-O'Donnell
John D. Aitchison
Barbara Knoblach
John Boyle
Source :
The Journal of Cell Biology
Publication Year :
2008
Publisher :
Rockefeller University Press, 2008.

Abstract

Reversible phosphorylation is the most common posttranslational modification used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evaluate signaling molecule deletion strains of the yeast Saccharomyces cerevisiae for presence of a green fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome functionality. We find that distinct signaling networks involving glucose-mediated gene repression, derepression, oleate-mediated induction, and peroxisome formation promote stages of the biogenesis pathway. Additionally, separate classes of signaling proteins are responsible for the regulation of peroxisome number and size. These signaling networks specify the requirements of early and late events of peroxisome biogenesis. Among the numerous signaling proteins involved, Pho85p is exceptional, with functional involvements in both gene expression and peroxisome formation. Our study represents the first global study of signaling networks regulating the biogenesis of an organelle.

Details

ISSN :
15408140 and 00219525
Volume :
181
Database :
OpenAIRE
Journal :
Journal of Cell Biology
Accession number :
edsair.doi.dedup.....b6f898f4658c01a4f9f77766c04b100b
Full Text :
https://doi.org/10.1083/jcb.200710009