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Genome-wide analysis of signaling networks regulating fatty acid–induced gene expression and organelle biogenesis
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2008
- Publisher :
- Rockefeller University Press, 2008.
-
Abstract
- Reversible phosphorylation is the most common posttranslational modification used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evaluate signaling molecule deletion strains of the yeast Saccharomyces cerevisiae for presence of a green fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome functionality. We find that distinct signaling networks involving glucose-mediated gene repression, derepression, oleate-mediated induction, and peroxisome formation promote stages of the biogenesis pathway. Additionally, separate classes of signaling proteins are responsible for the regulation of peroxisome number and size. These signaling networks specify the requirements of early and late events of peroxisome biogenesis. Among the numerous signaling proteins involved, Pho85p is exceptional, with functional involvements in both gene expression and peroxisome formation. Our study represents the first global study of signaling networks regulating the biogenesis of an organelle.
- Subjects :
- Green Fluorescent Proteins
Saccharomyces cerevisiae
Reviews
Biology
Article
Cell Physiological Phenomena
03 medical and health sciences
Genes, Reporter
Gene Expression Regulation, Fungal
Organelle
Peroxisomes
Animals
Research Articles
Derepression
030304 developmental biology
Organelles
0303 health sciences
Fatty Acids
Comment
030302 biochemistry & molecular biology
Cell Biology
Peroxisome
biology.organism_classification
Cell biology
Eukaryotic Cells
Gene Expression Regulation
Biochemistry
Phosphorylation
Organelle biogenesis
Genome, Fungal
Signal transduction
Gene Deletion
Biogenesis
Signal Transduction
Subjects
Details
- ISSN :
- 15408140 and 00219525
- Volume :
- 181
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....b6f898f4658c01a4f9f77766c04b100b
- Full Text :
- https://doi.org/10.1083/jcb.200710009