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RNA-Binding Proteins Revisited - The Emerging Arabidopsis mRNA Interactome

Authors :
Tino Köster
Dorothee Staiger
Katja Meyer
Claudius Marondedze
Source :
Trends in plant science. 22(6)
Publication Year :
2016

Abstract

RNA-protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture - where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.

Details

ISSN :
18784372
Volume :
22
Issue :
6
Database :
OpenAIRE
Journal :
Trends in plant science
Accession number :
edsair.doi.dedup.....b6b609c2e8fb8a9f6b32af077c61a125