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Increasing Uniformity of Biosurfactant Production in Starmerella bombicola via the Expression of Chimeric Cytochrome P450s
- Source :
- Colloids and Interfaces, Volume 2, Issue 4, Colloids and Interfaces, Vol 2, Iss 4, p 42 (2018), COLLOIDS AND INTERFACES
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Sophorolipids are one of the best known microbial biosurfactants and are produced by several yeast species. The best studied producer is Starmerella bombicola, a non-pathogenic yeast associated in nature with bumblebees. Sophorolipids are built up of the rare disaccharide sophorose, which is attached to a fatty acid through a glyosidic bound. Sophorolipids produced by S. bombicola mainly contain oleic acid as the incorporated hydrophobic group. Other chain lengths can, to a certain content, be incorporated by feeding the yeast with substrates of alternative chain lengths. However, the efficiency for such substrates is low as compared to the preferred C18 chain length and defined by the substrate specificity of the first enzymatic step in sophorolipid biosynthesis, i.e., the cytochrome P450 enzyme CYP52M1. To increase product uniformity and diversity at the same time, a new strain of S. bombicola was developed that produces sophorolipids with a palmitic acid acyl chain. This was achieved by heterologous expression of the cytochrome P450 cyp1 gene of Ustilago maydis and feeding with palmitic acid. Optimization of the production was done by protein and process engineering.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Sophorose
purification
BIOSYNTHETIC GENE-CLUSTER
SOPHOROLIPIDS
cytochrome P450
BM-3
Starmerella bombicola
01 natural sciences
Palmitic acid
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
Colloid and Surface Chemistry
process engineering
010608 biotechnology
fermentation
chemistry.chemical_classification
SOPHOROSE LIPID PRODUCTION
FERMENTATION
IDENTIFICATION
Sophorolipid
Fatty acid
Biology and Life Sciences
biosurfactant
REGIOSELECTIVITY
chimeragenesis
Yeast
NMR
Oleic acid
sophorolipid
enzyme engineering
030104 developmental biology
chemistry
Biochemistry
lcsh:QD1-999
Chemistry (miscellaneous)
Fermentation
Heterologous expression
MICROBIAL-PRODUCTION
Subjects
Details
- Language :
- English
- ISSN :
- 25045377
- Database :
- OpenAIRE
- Journal :
- Colloids and Interfaces
- Accession number :
- edsair.doi.dedup.....b6b424450f8ae2d38f557a9d6585313a
- Full Text :
- https://doi.org/10.3390/colloids2040042