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Increasing Uniformity of Biosurfactant Production in Starmerella bombicola via the Expression of Chimeric Cytochrome P450s

Authors :
Inge N. A. Van Bogaert
Marilyn De Graeve
Sophie Roelants
Robin Geys
Sofie De Maeseneire
Christophe Lemmens
Wim Soetaert
Jeroen Van Malderen
Christian V. Stevens
Sofie Lodens
Stein Mincke
Margaux De Smet
Source :
Colloids and Interfaces, Volume 2, Issue 4, Colloids and Interfaces, Vol 2, Iss 4, p 42 (2018), COLLOIDS AND INTERFACES
Publication Year :
2018
Publisher :
Multidisciplinary Digital Publishing Institute, 2018.

Abstract

Sophorolipids are one of the best known microbial biosurfactants and are produced by several yeast species. The best studied producer is Starmerella bombicola, a non-pathogenic yeast associated in nature with bumblebees. Sophorolipids are built up of the rare disaccharide sophorose, which is attached to a fatty acid through a glyosidic bound. Sophorolipids produced by S. bombicola mainly contain oleic acid as the incorporated hydrophobic group. Other chain lengths can, to a certain content, be incorporated by feeding the yeast with substrates of alternative chain lengths. However, the efficiency for such substrates is low as compared to the preferred C18 chain length and defined by the substrate specificity of the first enzymatic step in sophorolipid biosynthesis, i.e., the cytochrome P450 enzyme CYP52M1. To increase product uniformity and diversity at the same time, a new strain of S. bombicola was developed that produces sophorolipids with a palmitic acid acyl chain. This was achieved by heterologous expression of the cytochrome P450 cyp1 gene of Ustilago maydis and feeding with palmitic acid. Optimization of the production was done by protein and process engineering.

Details

Language :
English
ISSN :
25045377
Database :
OpenAIRE
Journal :
Colloids and Interfaces
Accession number :
edsair.doi.dedup.....b6b424450f8ae2d38f557a9d6585313a
Full Text :
https://doi.org/10.3390/colloids2040042