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Collagen V and vascular injury promote lung architectural changes in systemic sclerosis
- Source :
- The clinical respiratory journal. 3(3)
- Publication Year :
- 2010
-
Abstract
- Background: Systemic sclerosis (SSc) is a multisystem disorder characterized by inflammation, fibrosis and vascular damage. The aim of this study was to evaluate the interactions between basement membrane disruption, endothelial injury and collagen V deposition on the vascular wall, as well as their association with pulmonary function tests in patients with SSc. Method: The endothelial apoptosis was assessed by TUNEL and electron microscopy, and quantified through the point-counting technique. To evaluate basement membrane integrity, laminin immunostaining and electron microscopy were used. Immunofluorescence and morphometric analysis were used to determine the amount of collagen V in the vascular walls in 23 open lung biopsies of patients with SSc without pulmonary hypertension. Normal lung tissue was obtained from five individuals who had died of traumatic injuries. Results: The apoptosis index in SSc was higher in the endothelial cells (13.83 ± 6.83) when compared with the control (2.51 ± 2.06) group (P
- Subjects :
- Pulmonary and Respiratory Medicine
Adult
Male
Pathology
medicine.medical_specialty
Fluorescent Antibody Technique
Apoptosis
Basement Membrane
Pulmonary function testing
Pathogenesis
Immunoenzyme Techniques
Laminin
Fibrosis
medicine
In Situ Nick-End Labeling
Immunology and Allergy
Humans
Lung
Genetics (clinical)
Basement membrane
Analysis of Variance
Scleroderma, Systemic
biology
business.industry
Endothelial Cells
Middle Aged
medicine.disease
Pulmonary hypertension
Respiratory Function Tests
medicine.anatomical_structure
biology.protein
Female
Collagen
business
Immunostaining
Subjects
Details
- ISSN :
- 1752699X
- Volume :
- 3
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The clinical respiratory journal
- Accession number :
- edsair.doi.dedup.....b69dabbdaf9e2469dafa0ce183b3ecf2