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A conserved sequence motif in the E. coli soluble FAD-containing pyridine nucleotide transhydrogenase is important for reaction efficiency
- Source :
- The Journal of Biological Chemistry, 298(9):102304. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Publication Year :
- 2022
-
Abstract
- Soluble pyridine nucleotide transhydrogenases (STHs) are flavoenzymes involved in the redox homeostasis of the essential cofactors NAD(H) and NADP(H). They catalyze the reversible transfer of reducing equivalents between the two nicotinamide cofactors. The soluble transhydrogenase from Escherichia coli (SthA) has found wide use in both in vivo and in vitro applications to steer reducing equivalents toward NADPH-requiring reactions. However, mechanistic insight into SthA function is still lacking. In this work, we present a biochemical characterization of SthA, focusing for the first time on the reactivity of the flavoenzyme with molecular oxygen. We report on oxidase activity of SthA that takes place both during transhydrogenation and in the absence of an oxidized nicotinamide cofactor as an electron acceptor. We find that this reaction produces the reactive oxygen species hydrogen peroxide and superoxide anion. Furthermore, we explore the evolutionary significance of the well-conserved CXXXXT motif that distinguishes STHs from the related family of flavoprotein disulfide reductases in which a CXXXXC motif is conserved. Our mutational analysis revealed the cysteine and threonine combination in SthA leads to better coupling efficiency of transhydrogenation and reduced reactive oxygen species release compared to enzyme variants with mutated motifs. These results expand our mechanistic understanding of SthA by highlighting reactivity with molecular oxygen and the importance of the evolutionarily conserved sequence motif.
- Subjects :
- Niacinamide
Threonine
Nicotinamide Cofactors
Flavoproteins
Escherichia coli Proteins
Amino Acid Motifs
Hydrogen Peroxide
Cell Biology
NAD
Protein Engineering
Biochemistry
Oxygen
Superoxides
flavoprotein
NADP Transhydrogenase, B-Specific
Soluble Transhydrogenase
Amino Acid Sequence
Cysteine
Reactive Oxygen Species
Molecular Biology
Conserved Sequence
NADP
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 298
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- The Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....b69b00a35573d1e01a78c43692d9b50b