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Surface properties and intracellular speciation revealed an original adaptive mechanism to arsenic in the acid mine drainage bio-indicator Euglena mutabilis
- Source :
- Applied Microbiology and Biotechnology, Applied Microbiology and Biotechnology, Springer Verlag, 2011, pp.first online. ⟨10.1007/s00253-011-3493-y⟩
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- International audience; Euglena mutabilis is a protist ubiquitously found in extreme environments such as acid mine drainages which are often rich in arsenic. The response of E. mutabilis to this metalloid was compared to that of Euglena gracilis, a protist not found in such environments. Membrane fatty acid composition, cell surface properties, arsenic accumulation kinetics, and intracellular arsenic speciation were determined. The results revealed a modification in fatty acid composition leading to an increased membrane fluidity in both Euglena species under sublethal arsenic concentrations exposure. This increased membrane fluidity correlated to an induced gliding motility observed in E. mutabilis in the presence of this metalloid but did not affect the flagellar dependent motility of E. gracilis. Moreover, when compared to E. gracilis, E. mutabilis showed highly hydrophobic cell surface properties and a higher tolerance to water-soluble arsenical compounds but not to hydrophobic ones. Finally, E. mutabilis showed a lower accumulation of total arsenic in the intracellular compartment and an absence of arsenic methylated species in contrast to E. gracilis. Taken together, our results revealed the existence of a specific arsenical response of E. mutabilis that may play a role in its hypertolerance to this toxic metalloid.
- Subjects :
- inorganic chemicals
Euglena gracilis
Membrane Fluidity
Surface Properties
Gliding motility
[SDE.MCG]Environmental Sciences/Global Changes
Hydrophobicity
ved/biology.organism_classification_rank.species
chemistry.chemical_element
AMD
010501 environmental sciences
Biology
01 natural sciences
Applied Microbiology and Biotechnology
Euglena
Arsenic
03 medical and health sciences
[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry
Membrane fluidity
Soil Pollutants
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
030304 developmental biology
0105 earth and related environmental sciences
chemistry.chemical_classification
0303 health sciences
ved/biology
Cell Membrane
Fatty Acids
Euglena mutabilis
Fatty acid
Drug Tolerance
General Medicine
biology.organism_classification
Adaptation, Physiological
Unsaturation index
Biochemistry
chemistry
Arsenic speciation
[SDE]Environmental Sciences
Metalloid
Hydrophobic and Hydrophilic Interactions
Locomotion
Intracellular
Biotechnology
Subjects
Details
- ISSN :
- 14320614 and 01757598
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....b696e4d2eeb49267b5cf5e89676dd16a