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Molecular cloning and expression of the osteoclast-stimulating-factor-like gene from the rice field eel
- Source :
- Journal of experimental zoology. Part B, Molecular and developmental evolution. 302(2)
- Publication Year :
- 2004
-
Abstract
- Sexual development in vertebrates is a complex process. Vertebrates use several mechanisms to determine the development of a male or female organism. The genes for determination of sequential hermaphrodite sex are unknown. We identified a homologue of human osteoclast-stimulating factor (OSF) in the rice field eel, a teleost that undergoes natural sex transformation from female, via intersex, to male during its lifetime. The rice field eel OSF-like gene cDNA encoded a peptide of 214 amino acids that contains a c-Src homology 3 domain, proline-rich region, and ankyrin repeats, suggesting potential involvement in cell signaling. The gene was clustered into the OSF gene group of all the other vertebrates. Although expressed in the three kinds of gonads and in other tissues, OSF-like gene expression in gonads of all the three sexes was restricted to the gonadal germinal epithelium, from where bipotential gonia (oogonia or spermatogonia) will differentiate, suggesting that the OSF-like gene may be involved in sexual differentiation, in addition to its other roles as a regulator in development.
- Subjects :
- endocrine system
DNA, Complementary
Molecular Sequence Data
Biology
Homology (biology)
Hermaphrodite
Gene expression
Animals
Amino Acid Sequence
Gonads
Gene
In Situ Hybridization
Phylogeny
DNA Primers
Genetics
Expressed Sequence Tags
Expressed sequence tag
Sexual differentiation
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Intracellular Signaling Peptides and Proteins
General Medicine
Sequence Analysis, DNA
Blotting, Northern
Smegmamorpha
Gene expression profiling
Blotting, Southern
Testis determining factor
Animal Science and Zoology
Peptides
Sequence Alignment
Subjects
Details
- ISSN :
- 15525007
- Volume :
- 302
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of experimental zoology. Part B, Molecular and developmental evolution
- Accession number :
- edsair.doi.dedup.....b68418d41f889798a8fc34deb0eed46e