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Bovine milk-derived exosomes enhance goblet cell activity and prevent the development of experimental necrotizing enterocolitis
- Source :
- PLoS ONE, Vol 14, Iss 1, p e0211431 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Necrotizing enterocolitis (NEC) is characterized by intestinal injury and impaired mucin synthesis. We recently showed that breast milk exosomes from rodents promote intestinal cell viability, epithelial proliferation, and stem cell activity, but whether they also affect mucus production is unknown. Therefore, the aim of this study was to investigate the effects of bovine milk-derived exosomes on goblet cell expression in experimental NEC and delineate potential underlying mechanisms of action. Exosomes were isolated from bovine milk by ultracentrifugation and confirmed by Nanoparticle Tracking Analysis and through the detection of exosome membrane markers. To study the effect on mucin production, human colonic LS174T cells were cultured and exposed to exosomes. Compared to control, exosomes promoted goblet cell expression, as demonstrated by increased mucin production and relative expression levels of goblet cell expression markers trefoil factor 3 (TFF3) and mucin 2 (MUC2). In addition, exosome treatment enhanced the expression of glucose-regulated protein 94 (GRP94), the most abundant intraluminal endoplasmic reticulum (ER) chaperone protein that aids in protein synthesis. Furthermore, experimental NEC was induced in mouse pups by hyperosmolar formula feeding, lipopolysaccharide administration and hypoxia exposure on postnatal days 5-9. Milk exosomes were given with each gavage feed. NEC was associated with ileal morphological injury and reduction in MUC2+ goblet cells and GRP94+ cells per villus. Exosome administration to NEC pups prevented these changes. This research highlights the potential novel application of milk-derived exosomes in preventing the development of NEC in high-risk infants when breast milk is not available.
- Subjects :
- 0301 basic medicine
Physiology
Mucin 2
Exosomes
Endoplasmic Reticulum
Biochemistry
0302 clinical medicine
Spectrum Analysis Techniques
Medicine and Health Sciences
Intestinal Mucosa
Breast Milk
Multidisciplinary
Secretory Pathway
medicine.diagnostic_test
Chemistry
Trefoil factor 3
Flow Cytometry
Cell biology
Body Fluids
medicine.anatomical_structure
Milk
Cell Processes
Spectrophotometry
030220 oncology & carcinogenesis
Medicine
Female
Cytophotometry
Goblet Cells
Stem cell
Cellular Structures and Organelles
Anatomy
Research Article
Science
Research and Analysis Methods
Exosome
Flow cytometry
Beverages
03 medical and health sciences
Ileum
Enterocolitis, Necrotizing
medicine
Animals
Humans
Vesicles
Nutrition
Goblet cell
Milk, Human
Mucin
Biology and Life Sciences
Proteins
Cell Biology
Microvesicles
digestive system diseases
Diet
Gastrointestinal Tract
Mice, Inbred C57BL
030104 developmental biology
Cattle
Digestive System
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b672635dfb2d97a69933adf1833b68a7