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Ultrasound-assisted compatible in situ hydrolysis of sugarcane bagasse in cellulase-aqueous–N-methylmorpholine-N-oxide system for improved saccharification
- Source :
- Bioresource Technology. 107:251-257
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- To fully exploit the benefits of N-methylmorpholine-N-oxide (NMMO) in lignocelluloses bioconversion, a compatible system was established for efficient in situ saccharification of cellulose in NMMO-aqueous media in which the NMMO is able to activate and solubilize the cellulose, and the cellulases possess high stability and activity. Cellulase retained its original activity after being pre-incubated in 15% and 20% (w/v) NMMO solutions. After optimization of reaction parameters, high saccharification rate (96.5%) was obtained in aqueous-NMMO media by ultrasound assisted treatment of cellulose. The viscosity and FTIR analysis revealed that NMMO-treated cellulose under ultrasonic condition was porous and amorphous, which led to improved saccharification. The addition of trifle lignin in lower concentration improved the saccharification efficiency of sugarcane bagasse, while higher concentration interferes with hydrolysis. In conclusion, these findings provided great implications to develop a continuous process NMMO-cellulases system for transformation of native biomass.
- Subjects :
- Environmental Engineering
Bioconversion
Morpholines
Carbohydrates
Bioengineering
Cellulase
Cyclic N-Oxides
Hydrolysis
chemistry.chemical_compound
Enzymatic hydrolysis
Spectroscopy, Fourier Transform Infrared
Lignin
Ultrasonics
Cellulose
Waste Management and Disposal
Waste management
biology
Viscosity
Renewable Energy, Sustainability and the Environment
Chemistry
N-Methylmorpholine N-oxide
General Medicine
Saccharum
Chemical engineering
biology.protein
Bagasse
Subjects
Details
- ISSN :
- 09608524
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology
- Accession number :
- edsair.doi.dedup.....b671facc629dcf3321157f4ed672793a
- Full Text :
- https://doi.org/10.1016/j.biortech.2011.12.068