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SYK and LYN Mediate B-cell Receptor-independent Calcium-induced Apoptosis in DT-40 Lymphoma B-cells
- Source :
- Leukemia & Lymphoma. 43:2165-2170
- Publication Year :
- 2002
- Publisher :
- Informa UK Limited, 2002.
-
Abstract
- Here, we report that the calcium ionophore ionomycin induces a massive Ca2+-dependent apoptosis in wildtype DT-40 chicken B lymphoma cells, as well as in BTK-deficient, PLCgamma2-deficient and IP3 receptor-deficient DT-40 cells, but not in LYN- or SYK-deficient DT-40 cells. Notably, the deficiency of CSK, a negative regulator of Src-family PTK, promoted ionomycin-induced apoptosis of DT-40 cells. Reconstitution of SYK-deficient cells with wild-type SYK restored the apoptotic response of the cells to ionomycin, but the expression of FYN or LCK in LYN-deficient cells did not restore the apoptotic response of LYN-deficient cells. Taken together, our data suggests that both LYN and SYK, but not BTK, FYN or LCK, are crucial mediators of BCR-independent Ca2+-induced apoptosis in DT-40 lymphoma B cells.
- Subjects :
- Cancer Research
Lymphoma, B-Cell
B-cell receptor
Ionophore
Receptors, Antigen, B-Cell
Syk
chemistry.chemical_element
Apoptosis
Calcium
Proto-Oncogene Proteins c-fyn
chemistry.chemical_compound
immune system diseases
LYN
Proto-Oncogene Proteins
hemic and lymphatic diseases
Agammaglobulinaemia Tyrosine Kinase
Tumor Cells, Cultured
medicine
Animals
Syk Kinase
B-Lymphocytes
Enzyme Precursors
Chemistry
Ionomycin
Intracellular Signaling Peptides and Proteins
Hematology
Protein-Tyrosine Kinases
medicine.disease
Lymphoma
src-Family Kinases
Oncology
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Cancer research
Chickens
Subjects
Details
- ISSN :
- 10292403 and 10428194
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Leukemia & Lymphoma
- Accession number :
- edsair.doi.dedup.....b6502564435409362d5044f082a90f0e