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The diagnostic utility of EZH2 H-score and Ki-67 index in non-invasive breast apocrine lesions
- Source :
- Pathology, research and practice. 216(9)
- Publication Year :
- 2020
-
Abstract
- In diagnostic breast pathology, there is no reliable applicable immunostain to help discern atypical and in situ apocrine lesions from benign apocrine tissue. At present, the diagnosis of non-invasive apocrine lesions remains challenging with current diagnoses rendered based on discrete morphologic characteristics on conventional hematoxylin and eosin staining. Interobserver variability is significant even among subspecialists partly due to lack of adjuvant diagnostic immunohistochemical stains. Herein, we set to elucidate the potential utility of EZH2 and Ki-67 immunostains as tangible tools in non-invasive apocrine proliferations. A cohort of apocrine breast lesions [Benign apocrine hyperplasia (BAH), n = 10; Atypical apocrine hyperplasia (AAH), n = 16; Apocrine ductal carcinoma in situ (ADCIS), n = 12] were subjected to EZH2 immunostaining and analyzed via H-scoring of nuclear expression. Mean H-scores for EZH2 progressively increased from BAH (23.5), to AAH (47.4) and ADCIS (196.4), and showed a significant difference utilizing the Kruskal-Wallis test (p < 0.0001). Further interrogation of Ki-67 demonstrated incremental expression from BAH to AAH and ADCIS at 1.6 %, 4.7 % and 24.7 %, respectively (p < 0.0001, Kruskal-Wallis test), suggesting an association with increased proliferation. Our results demonstrate that a combination of EZH2 and Ki-67 immunostaining may be employed in differentiating among challenging apocrine breast lesions and suggest a putative diagnostic utility for EZH2 and Ki-67 in non-invasive apocrine breast lesions.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
H&E stain
Breast Neoplasms
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Atypia
medicine
Biomarkers, Tumor
Humans
Enhancer of Zeste Homolog 2 Protein
Breast
Metaplasia
Hyperplasia
biology
business.industry
Apocrine
Epithelial Cells
Cell Biology
Ductal carcinoma
medicine.disease
030104 developmental biology
Apocrine Glands
Ki-67 Antigen
030220 oncology & carcinogenesis
Ki-67
biology.protein
Immunohistochemistry
Female
business
Precancerous Conditions
Immunostaining
Subjects
Details
- ISSN :
- 16180631
- Volume :
- 216
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Pathology, research and practice
- Accession number :
- edsair.doi.dedup.....b64bbf0a13ef11cb8a44b2d1bf0ca085