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Daptomycin and rifampin alone and in combination prevent vascular graft biofilm formation and emergence of antibiotic resistance in a subcutaneous rat pouch model of staphylococcal infection
- Publication Year :
- 2010
-
Abstract
- Objective To investigate the efficacy of daptomycin and rifampin either alone or in combination in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Design Prospective, randomised, controlled animal study. Materials Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2×10 7 colony forming units of Staphylococcus aureus , strain Smith diffuse. Methods The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and three contaminated groups that received intra-peritoneal daptomycin, rifampin-soaked graft and daptomycin plus rifampin-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro antibiotic susceptibility assay for S. aureus biofilms was performed to elucidate the same activity. Results When tested alone, daptomycin and rifampin showed good efficacies. Their combination showed efficacies significantly higher than that of each single compound. The in vitro studies showed that Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values for daptomycin were lower in presence of rifampin. Daptomycin prevented the emergence of rifampin resistance. Conclusion Daptomycin is an important candidate for prevention of staphylococcal biofilm-related infection and rifampin could serve as an interesting anti-staphylococcal antibiotic enhancer.
- Subjects :
- Male
Staphylococcus aureus
Prosthesis-Related Infections
medicine.drug_class
Vascular graft infection
Antibiotics
Colony Count, Microbial
Microbial Sensitivity Tests
Drug resistance
Prosthesis Design
medicine.disease_cause
Microbiology
Blood Vessel Prosthesis Implantation
Random Allocation
Minimum inhibitory concentration
Lipopeptides
Antibiotic resistance
Coated Materials, Biocompatible
Daptomycin
Drug Resistance, Multiple, Bacterial
medicine
polycyclic compounds
Animals
Prospective Studies
Rats, Wistar
Antibiotic prophylaxis
Bacterial biofilm
Medicine(all)
Minimum bactericidal concentration
Polyethylene Terephthalates
business.industry
Antibiotic Prophylaxis
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
Anti-Bacterial Agents
Blood Vessel Prosthesis
Rats
Disease Models, Animal
Biofilms
Drug Therapy, Combination
Surgery
Rifampin
Cardiology and Cardiovascular Medicine
business
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....b62911ff04f0ad4a681119b474c235c8