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Emulsification efficiency of adsorbed chitosan for bacterial cells accumulation at the oil–water interface

Emulsification efficiency of adsorbed chitosan for bacterial cells accumulation at the oil–water interface

Authors :
Somruethai Khamsakhon
Somwit Archakunakorn
Nattapat Charoenrat
Jirarut Wongkongkatep
Pravit Wongkongkatep
Manop Suphantharika
Thunyarat Pongtharangkul
Source :
Bioprocess and Biosystems Engineering. 38:701-709
Publication Year :
2014
Publisher :
Springer Science and Business Media LLC, 2014.

Abstract

The use of bacterial cell or biocatalyst for industrial synthetic chemistry is on the way of significant growth since the biocatalyst requires low energy input compared to the chemical synthesis and can be considered as a green technology. However, majority of natural bacterial cell surface is hydrophilic which allows poor access to the hydrophobic substrate or product. In this study, Escherichia coli (E. coli) as a representative of hydrophilic bacterial cells were accumulated at the oil–water interface after association with chitosan at a concentration range of 0.75–750 mg/L. After association with negatively charged E coli having a ζ potential of −19.9 mV, a neutralization of positively charged chitosan occurred as evidenced by an increase in the ζ potential value of the mixtures with increasing chitosan concentration up to +3.5 mV at 750 mg/L chitosan. Both emulsification index and droplet size analysis revealed that chitosan-E. coli system is an excellent emulsion stabilizer to date because the threshold concentration was as low as 7.5 mg/L or 0.00075 % w/v. A dramatic increase in the surface hydrophobicity of the E. coli as evidenced by an increase in contact angle from 19 to 88° with increasing chitosan concentration from 0 to 750 mg/L, respectively, resulted in an increase in the stability of oil-in-water emulsions stabilized by chitosan-E. coli system. The emulsion was highly stable even the emulsification was performed under 20 % salt condition, or temperature ranged between 20 and 50 °C. Emulsification was failed when the oil volume fraction was higher than 0.5, indicating that no phase inversion occurred. The basic investigation presented in this study is a crucial platform for its application in biocatalyst industry and bioremediation of oil spill.

Details

ISSN :
16157605 and 16157591
Volume :
38
Database :
OpenAIRE
Journal :
Bioprocess and Biosystems Engineering
Accession number :
edsair.doi.dedup.....b5e54c573077e3fd254d8a451ea39731
Full Text :
https://doi.org/10.1007/s00449-014-1310-6