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Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research
- Source :
- Molecules and Cells
- Publication Year :
- 2017
- Publisher :
- Korean Society for Molecular and Cellular Biology, 2017.
-
Abstract
- Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.
- Subjects :
- 0301 basic medicine
transcriptional activator-like effector
Biomedical Research
Computer science
Computational biology
Protein Engineering
Bioinformatics
DNA sequencing
Genome engineering
Domain (software engineering)
03 medical and health sciences
Animals
Humans
Amino Acid Sequence
sequence-specific DNA detection
Molecular Biology
Regulation of gene expression
Zinc finger
Flexibility (engineering)
Base Sequence
Effector
Gene targeting
Zinc Fingers
DNA
Cell Biology
General Medicine
DNA-Binding Proteins
030104 developmental biology
Trans-Activators
biomedical application
Minireview
Subjects
Details
- ISSN :
- 02191032 and 10168478
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Molecules and Cells
- Accession number :
- edsair.doi.dedup.....b5dc3a78d3e76dec37b85f207a518145