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Efficient Generation of Fully Reprogrammed Human iPS Cells via Polycistronic Retroviral Vector and a New Cocktail of Chemical Compounds
- Source :
- PLoS ONE, PLoS ONE, Vol 6, Iss 10, p e26592 (2011)
- Publication Year :
- 2011
- Publisher :
- Public Library of Science, 2011.
-
Abstract
- Direct reprogramming of human somatic cells into induced pluripotent stem (iPS) cells by defined transcription factors (TFs) provides great potential for regenerative medicine and biomedical research. This procedure has many challenges, including low reprogramming efficiency, many partially reprogrammed colonies, somatic coding mutations in the genome, etc. Here, we describe a simple approach for generating fully reprogrammed human iPS cells by using a single polycistronic retroviral vector expressing four human TFs in a single open reading frame (ORF), combined with a cocktail containing three small molecules (Sodium butyrate, SB431542, and PD0325901). Our results demonstrate that human iPS cells generated by this approach express human ES cells markers and exhibit pluripotency demonstrated by their abilities to differentiate into the three germ layers in vitro and in vivo. Notably, this approach not only provides a much faster reprogramming process but also significantly diminishes partially reprogrammed iPS cell colonies, thus facilitating efficient isolation of desired fully reprogrammed iPS cell colonies.
- Subjects :
- Somatic cell
Cellular differentiation
Induced Pluripotent Stem Cells
Genetic Vectors
Cell Culture Techniques
lcsh:Medicine
Dioxoles
Biology
Regenerative medicine
Viral vector
03 medical and health sciences
0302 clinical medicine
Humans
Induced pluripotent stem cell
lcsh:Science
Embryonic Stem Cells
030304 developmental biology
Genetics
0303 health sciences
Multidisciplinary
Stem Cells
lcsh:R
Diphenylamine
Cellular Reprogramming
Embryonic stem cell
3. Good health
Cell biology
Butyrates
Retroviridae
Cell culture
Small Molecules
Benzamides
lcsh:Q
Reprogramming
Receptors, Transforming Growth Factor beta
030217 neurology & neurosurgery
Research Article
Biotechnology
Developmental Biology
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b5ccf42ee23128ab5bba2f0bcca000ae