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Green tea proanthocyanidins cause impairment of hormone-regulated larval development and reproductive fitness via repression of juvenile hormone acid methyltransferase, insulin-like peptide and cytochrome P450 genes in Anopheles gambiae sensu stricto
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 3, p e0173564 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- Successful optimization of plant-derived compounds into control of nuisance insects would benefit from scientifically validated targets. However, the close association between the genotypic responses and physiological toxicity effects mediated by these compounds remains underexplored. In this study, we evaluated the sublethal dose effects of proanthocyanidins (PAs) sourced from green tea (Camellia sinensis) on life history traits of Anopheles gambiae (sensu stricto) mosquitoes with an aim to unravel the probable molecular targets. Based on the induced phenotypic effects, genes selected for study targeted juvenile hormone (JH) biosynthesis, signal transduction, oxidative stress response and xenobiotic detoxification in addition to vitellogenesis in females. Our findings suggest that chronic exposure of larval stages (L3/L4) to sublethal dose of 5 ppm dramatically extended larval developmental period for up to 12 days, slowed down pupation rates, induced abnormal larval-pupal intermediates and caused 100% inhibition of adult emergence. Further, females exhibited significant interference of fecundity and egg hatchability relative to controls (p < 0.001). Using reverse transcription quantitative polymerase chain reaction (RT-qPCR), our findings show that PA-treated larvae exhibited significant repression of AgamJHAMT (p < 0.001), AgamILP1 (p < 0.001) and AgamCYP6M2 (p < 0.001) with up-regulation of Hsp70 (p < 0.001). Females exposed as larvae demonstrated down-regulation of AgamVg (p = 0.03), AgamILP1 (p = 0.009), AgamCYP6M2 (p = 0.05) and AgamJHAMT (p = 0.02). Our findings support that C. sinensis proanthocyanidins affect important vectorial capacity components such as mosquito survival rates and reproductive fitness thus could be potentially used for controlling populations of malaria vectors.
- Subjects :
- 0301 basic medicine
Life Cycles
Anopheles gambiae
lcsh:Medicine
Gene Expression
Disease Vectors
Toxicology
Pathology and Laboratory Medicine
Mosquitoes
Biochemistry
0302 clinical medicine
Larvae
Cytochrome P-450 Enzyme System
Gene expression
Medicine and Health Sciences
Insulin
lcsh:Science
Larva
Multidisciplinary
biology
Insects
Juvenile Hormones
Phenotypes
Infectious Diseases
Toxicity
Research Article
Arthropoda
030231 tropical medicine
Biosynthesis
Andrology
03 medical and health sciences
Anopheles
Genetics
Parasitic Diseases
Animals
Proanthocyanidins
Dose-Response Relationship, Drug
Tea
lcsh:R
Organisms
Cytochrome P450
Biology and Life Sciences
Methyltransferases
biology.organism_classification
Tropical Diseases
Molecular biology
Invertebrates
Hormones
Insect Vectors
Malaria
Species Interactions
030104 developmental biology
Juvenile hormone
biology.protein
lcsh:Q
Vitellogenesis
Hormone
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....b5c1bb481ef2373c1134555a6a758b0c