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Neutrophil P-selectin-glycoprotein-ligand-1 binding to platelet P-selectin enhances metalloproteinase 2 secretion and platelet-neutrophil aggregation
- Source :
- Thrombosis and Haemostasis. 94:1230-1235
- Publication Year :
- 2005
- Publisher :
- Georg Thieme Verlag KG, 2005.
-
Abstract
- SummaryPlatelets and neutrophils constitute a high source of metalloproteinases (MMPs), and their interactions via P-selectin and Pselectin- glycoprotein-ligand-1 (PSGL-1) are involved in thrombosis, vascular remodelling, and restenosis. We investigated the impact of these interactions on platelet MMP-2 secretion and function in platelet and neutrophil aggregation. The secretion of MMP-2 from human platelets was significantly increased threefold after thrombin activation, and enhanced two-fold in the presence of neutrophils. Neutrophil supernatant had no effect on platelet MMP-2 secretion. While no MMP-2 was detected in the supernatant of neutrophils, a high amount of MMP-9 was released by neutrophils, and remained unchanged upon thrombin activation or in the presence of platelets. Platelet P-selectin, which increased significantly after activation, triggered platelet binding to neutrophils that was completely inhibited by P-selectin or PSGL-1 antagonists, and was reduced by 50% with a GPIIb/ IIIa antagonist. P-selectin or PSGL-1 antagonism abolished the enhanced secretion of platelet MMP-2 in the presence of neutrophils and reduced platelet-neutrophil aggregation. Platelet activation and binding to neutrophils enhance the secretion of platelet MMP-2 via an adhesive interaction between P-selectin and PSGL-1, which contribute to increase platelet-neutrophil aggregation.
- Subjects :
- Blood Platelets
medicine.medical_specialty
P-selectin
Neutrophils
Cell Communication
In Vitro Techniques
030204 cardiovascular system & hematology
Granulocyte
Biology
Antibodies
03 medical and health sciences
Platelet Adhesiveness
0302 clinical medicine
Thrombin
Internal medicine
Cell Adhesion
medicine
Humans
Platelet
Platelet activation
Neutrophil aggregation
030304 developmental biology
0303 health sciences
Membrane Glycoproteins
Hematology
3. Good health
P-Selectin
Endocrinology
medicine.anatomical_structure
Matrix Metalloproteinase 9
Biochemistry
Matrix Metalloproteinase 2
P-selectin glycoprotein ligand-1
Platelet factor 4
medicine.drug
Subjects
Details
- ISSN :
- 2567689X and 03406245
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Thrombosis and Haemostasis
- Accession number :
- edsair.doi.dedup.....b5c0df7eafea3c3b8656958d34548958