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Reference intervals for lymphocyte subsets in preterm and term neonates without immune defects
- Source :
- The Journal of allergy and clinical immunology, vol 144, iss 6
- Publication Year :
- 2019
-
Abstract
- Background In 6.5 years of newborn screening for severe combined immunodeficiency in California, 3,252,156 infants had DNA from dried blood spots (DBSs) assayed for T-cell receptor excision circles. Infants with T-cell receptor excision circle values of less than a designated cutoff on a single DBS, 2 DBS samples with insufficient PCR amplification, or known genetic risk of immunodeficiency had peripheral blood complete blood counts and lymphocyte subsets assayed in a single flow cytometry laboratory. Cases in which immune defects were ruled out were available for analysis. Objective We sought to determine reference intervals for lymphocyte subsets in racially/ethnically diverse preterm and term newborns who proved to be unaffected by any T-lymphopenic immune disorder. Methods Effective gestational age (GA) was defined as GA at birth plus postnatal age at the time of sample collection. After determining exclusion criteria, we analyzed demographic and clinical information, complete and differential white blood cell counts, and lymphocyte subsets for 301 infants, with serial measurements for 33 infants. Lymphocyte subset measurements included total T cells, helper and cytotoxic T-cell subsets, naive and memory phenotype of each T-cell subset, B cells, and natural killer cells. Results Reference intervals were generated for absolute numbers and lymphocyte subsets from infants with effective GAs of 22 to 52 weeks. Sex and ethnicity were not significant determinants of lymphocyte subset counts in this population. Lymphocyte counts increased postnatally. Conclusion This study provides a baseline for interpreting comprehensive lymphocyte data in preterm and term infants, aiding clinicians to determine which newborns require further evaluations for immunodeficiency.
- Subjects :
- 0301 basic medicine
Male
Allergy
Helper-Inducer
Lymphocyte
T-Lymphocytes
reference range/reference interval
T-cell subsets
memory T cell
Reproductive health and childbirth
CD8-Positive T-Lymphocytes
Low Birth Weight and Health of the Newborn
Polymerase Chain Reaction
0302 clinical medicine
Receptors
Infant Mortality
Immunology and Allergy
Flow cytometry
Immunodeficiency
Pediatric
education.field_of_study
B-Lymphocytes
T-cell receptor excision circles
T-Lymphocytes, Helper-Inducer
severe combined immunodeficiency
medicine.anatomical_structure
Antigen
Female
Sample collection
Infant, Premature
Population
Immunology
Receptors, Antigen, T-Cell
Gestational Age
neonatal immunity
Vaccine Related
03 medical and health sciences
naive T cell
Clinical Research
Preterm
030225 pediatrics
White blood cell
medicine
Genetics
Humans
Lymphocyte Count
education
Premature
T-cell receptor excision circle
Newborn screening
business.industry
newborn screening
Inflammatory and immune system
Infant, Newborn
Infant
preterm birth
Perinatal Period - Conditions Originating in Perinatal Period
medicine.disease
T-Cell
Newborn
030104 developmental biology
Dried Blood Spot Testing
business
Memory T cell
Subjects
Details
- ISSN :
- 10976825
- Volume :
- 144
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Journal of allergy and clinical immunology
- Accession number :
- edsair.doi.dedup.....b5a4a5299386f7395f060639af31876f