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Effects of sialidase NEU1 siRNA on proliferation, apoptosis, and invasion in human ovarian cancer
- Source :
- Molecular and cellular biochemistry. 411(1-2)
- Publication Year :
- 2015
-
Abstract
- Ovarian cancer is one of the most common malignancies encountered in the world. In ovarian cancer tissues of patients, NEU1 was expressed in a higher level than that in adjacent normal tissues. In this research, we aimed to elucidate the role of NEU1 siRNA on proliferation, apoptosis, and invasion of OVCAR3 and SKOV3 cells which expressed NEU1 notably. By cell viability assay and flow cytometry method, we found that NEU1 siRNA effectively inhibited the cancer proliferation, arrested cells cycle at G0/G1 phase, and induced apoptosis when compared to the Mock group. Result of transwell assay showed that invasion of cells in OVCAR3 and SKOV3 treated with NEU1 siRNA were suppressed significantly. Gene set enrichment analysis showed that lysosome and oxidative phosphorylation related signal pathway were associated with the NEU1 expression. In addition, Western blot revealed that expressions of Cln3 and Cln5 were depressed, and ATP5B and ATP5J expressions were also reduced. In conclusion, NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment.
- Subjects :
- 0301 basic medicine
endocrine system diseases
ATP5B
Clinical Biochemistry
Neuraminidase
Apoptosis
Biology
Flow cytometry
03 medical and health sciences
Western blot
Pregnancy
Lysosome
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
Viability assay
RNA, Small Interfering
Molecular Biology
Cell Proliferation
Ovarian Neoplasms
medicine.diagnostic_test
Cancer
Cell Biology
General Medicine
medicine.disease
030104 developmental biology
medicine.anatomical_structure
Gene Knockdown Techniques
Immunology
Cancer research
Female
Ovarian cancer
Subjects
Details
- ISSN :
- 15734919
- Volume :
- 411
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biochemistry
- Accession number :
- edsair.doi.dedup.....b587cc3c75f6e48b5f4d81475c1f91c6