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An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP
- Source :
- FEBS Letters. 583:157-162
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.
- Subjects :
- Conformational change
Protein Conformation
Biophysics
Biosensing Techniques
Biochemistry
Maltose-Binding Proteins
Maltose-binding protein
chemistry.chemical_compound
Structural Biology
MBP
Genetics
Surface charge
Maltose
Molecular Biology
biology
Chemistry
Cell Biology
Semiconductors
biology.protein
Field-effect transistor
Target protein
ISFET
Carrier Proteins
Biosensor
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 583
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....b572ce1121f8ef25a3e385406556ff5c