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BC nanofibres: in vitro study of genotoxicity and cell proliferation
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2009
- Publisher :
- Elsevier, 2009.
-
Abstract
- Nanomaterials have unusual properties not found in the bulk materials, which can be exploited in numerous applications such as biosensing, electronics, scaffolds for tissue engineering, diagnostics and drug delivery. However, research in the past few years has turned up a range of potential health hazards, which has given birth to the new discipline of nanotoxicology. Bacterial cellulose (BC) is a promising material for biomedical applications, namely due its biocompatibility. Although BC has been shown not to be cytotoxic or genotoxic, the properties of isolated BC nanofibres (NFs) on cells and tissues has never been analysed. Considering the toxicity associated to other fibre-shaped nanoparticles, it seems crucial to evaluate the toxicity associated to the BC-NFs. In thiswork, nanofibreswere produced frombacterial cellulose by a combination of acid and ultrasonic treatment. The genotoxicity of nanofibres from bacterial cellulosewas analysed in vitro, using techniques previously demonstrated to detect the genotoxicity of fibrous nanoparticles. The results from single cell gel electrophoresis (also known as comet assay) and the Salmonella reversion assays showed that NFs are not genotoxicity under the conditions tested. A proliferation assay using fibroblasts and CHO cells reveals a slight reduction in the proliferation rate, although no modification in the cell morphology is observed.<br />Fundação para a Ciência e a Tecnologia (FCT)<br />Conselho Nacional de Desenvolvimento Científico e Tecnológico Brazil (CNPq)<br />CAPES Brazil (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
- Subjects :
- Salmonella typhimurium
Biocompatibility
Biocompatible Materials
CHO Cells
02 engineering and technology
Toxicology
medicine.disease_cause
Cell morphology
Microbiology
Bacterial cellulose
Mice
03 medical and health sciences
chemistry.chemical_compound
Cricetulus
Microscopy, Electron, Transmission
Tissue engineering
Cricetinae
medicine
Animals
Nonotoxicology
Cellulose
Cell Proliferation
030304 developmental biology
0303 health sciences
Science & Technology
Gluconacetobacter xylinus
3T3 Cells
General Medicine
Fibroblasts
021001 nanoscience & nanotechnology
Culture Media
3. Good health
Comet assay
Biochemistry
chemistry
Nanotoxicology
Drug delivery
Nanoparticles
Comet Assay
Genotoxicity
0210 nano-technology
Nanofibres
DNA Damage
Mutagens
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....b56ba0ab7e5150033dab0e73d6af5cbe