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Riboflavin induces Metarhizium spp. to produce conidia with elevated tolerance to UV-B, and upregulates photolyases, laccases and polyketide synthases genes
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2018
- Publisher :
- Wiley Blackwell Publishing, Inc, 2018.
-
Abstract
- Aims: The effect of nutritional supplementation of two Metarhizium species with riboflavin (Rb) during production of conidia was evaluated on (i) conidial tolerance (based on germination) to UV-B radiation and on (ii) conidial expression following UV-B irradiation, of enzymes known to be active in photoreactivation, viz., photolyase (Phr), laccase (Lac) and polyketide synthase (Pks). Methods and Results: Metarhizium acridum (ARSEF 324) and Metarhizium robertsii (ARSEF 2575) were grown either on (i) potato dextrose agar medium (PDA), (ii) PDA supplemented with 1% yeast extract (PDAY), (iii) PDA supplemented with Rb (PDA+Rb), or (iv) PDAY supplemented with Rb (PDAY+Rb). Resulting conidia were exposed to 866·7 mW m−2 of UV-B Quaite-weighted irradiance to total doses of 3·9 or 6·24 kJ m−2. Some conidia also were exposed to 16 klux of white light (WL) after being irradiated, or not, with UV-B to investigate the role of possible photoreactivation. Relative germination of conidia produced on PDA+Rb (regardless Rb concentration) or on PDAY and exposed to UV-B was higher compared to conidia cultivated on PDA without Rb supplement, or to conidia suspended in Rb solution immediately prior to UV-B exposure. The expression of MaLac3 and MaPks2 for M. acridum, as well as MrPhr2, MrLac1, MrLac2 and MrLac3 for M. robertsii was higher when the isolates were cultivated on PDA+Rb and exposed to UV-B followed by exposure to WL, or exposed to WL only. Conclusions: Rb in culture medium increases the UV-B tolerance of M. robertsii and M. acridum conidia, and which may be related to increased expression of Phr, Lac and Pks genes in these conidia. Significance and Impact of the Study: The enhanced UV-B tolerance of Metarhizium spp. conidia produced on Rb-enriched media may improve the effectiveness of these fungi in biological control programs.<br />Instituto de Investigaciones Bioquímicas de La Plata
- Subjects :
- Bioquímica
0301 basic medicine
PHOTOLYASE
Metarhizium
Ultraviolet Rays
Metarhizium acridum
Biología
Riboflavin
030106 microbiology
Photoreactivation
Applied Microbiology and Biotechnology
RIBOFLAVIN
TOLERANCE TO UV-B
Conidium
Microbiology
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
03 medical and health sciences
Polyketide
LACCASE
Polyketide synthase
Metarhizium robertsii
Photolyase
purl.org/becyt/ford/1.6 [https]
Gene
Laccase
Tolerance to UV-B
biology
PHOTOREACTIVATION
General Medicine
Spores, Fungal
Bioquímica y Biología Molecular
biology.organism_classification
METARHIZIUM ROBERTSII
Up-Regulation
POLYKETIDE SYNTHASE
030104 developmental biology
biology.protein
METARHIZIUM ACRIDUM
Deoxyribodipyrimidine Photo-Lyase
Polyketide Synthases
CIENCIAS NATURALES Y EXACTAS
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Accession number :
- edsair.doi.dedup.....b55693e42af66575f85948b6ed4735bf