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Duck PIAS2 negatively regulates RIG-I mediated IFN-β production by interacting with IRF7
- Source :
- Developmental and comparative immunology. 108
- Publication Year :
- 2019
-
Abstract
- The protein inhibitor of activated STAT (PIAS) proteins are important signal transduction modulator family and regulate the innate immune signaling pathway induced by certain transcription factors, including NF-κB, IRF3, and JAK/STAT. The PIAS protein mechanism that regulates innate immune response in mammals has been well described in the literature; however, whether the PIAS gene exists in ducks as well as the role of PIAS in duck IFN-β expression is still unclear. Here, we cloned duck PIAS (duPIAS), finding PIAS2 could repress IFN-β production. DuPIAS2 contains SAP-PINIT-RLD-S/T characteristic domains, and its overexpression could inhibit virus-induced IFN-β promoter activation. Moreover, duPIAS2 interacts with duck interferon regulatory factor 7 (IRF7) and inhibits IFN-β promoter activation induced by duck IRF7. Additionally, its inhibitory function does not rely on its SUMO E3 ligase activity but rather its C-terminal portion. The above results demonstrate that duPIAS2 is a repressor of IFN-β production induced by duck IRF7.
- Subjects :
- 0301 basic medicine
animal diseases
Interferon Regulatory Factor-7
Immunology
Repressor
Biology
03 medical and health sciences
0302 clinical medicine
Protein Domains
Animals
Humans
Protein inhibitor of activated STAT
Promoter Regions, Genetic
Transcription factor
Poultry Diseases
Interferon-beta
Vesiculovirus
Protein Inhibitors of Activated STAT
Immunity, Innate
Recombinant Proteins
Ubiquitin ligase
Cell biology
030104 developmental biology
Ducks
HEK293 Cells
Gene Expression Regulation
Influenza A virus
biology.protein
IRF7
DEAD Box Protein 58
Signal transduction
IRF3
030215 immunology
Developmental Biology
Interferon regulatory factors
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 18790089
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Developmental and comparative immunology
- Accession number :
- edsair.doi.dedup.....b555a7b74809ecc3e01e12872af8c293