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C-type natriuretic peptide/guanylate cyclase B system in ATDC5 cells, a chondrogenic cell line
- Source :
- Journal of bone and mineral metabolism. 20(3)
- Publication Year :
- 2002
-
Abstract
- Natriuretic peptides constitute a family of three structurally related peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Particulate guanylate cyclases, GC-A, and GC-B, are the receptors for these peptides to mediate their action. ANP and BNP possess high affinities for GC-A, and CNP is the preferred ligand for GC-B. In this article, we report our study of the expression and possible role(s) of natriuretic peptides in ATDC5 cells, which represent a chondrogenic cell line. ATDC5 cells produced cyclic guanosine monophosphate (cGMP) in response to natriuretic peptides. CNP was far more potent than ANP in terms of cGMP production. The messages for GC-A and GC-B were demonstrated by means of Northern blot analysis, and the presence of CNP was shown by Southern blotting coupled with reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that the CNP/GC-B system is preferentially expressed in ATDC5 cells. GC-B mRNA expression was higher at 14 days after confluency than that at confluency. CNP or 8-bromo cGMP reduced [3H] thymidine uptake and slightly increased the message for collagen type X, which is a marker of hypertrophic chondrocytes. These data suggest that the CNP/GC-B system is likely to be an autocrine/paracrine regulator of ATDC5 cells, thus affecting both their growth and differentiation.
- Subjects :
- DNA Replication
medicine.medical_specialty
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Biology
PC12 Cells
Cell Line
chemistry.chemical_compound
Mice
Endocrinology
Chondrocytes
Atrial natriuretic peptide
Internal medicine
medicine
Natriuretic peptide
Animals
Orthopedics and Sports Medicine
RNA, Messenger
Autocrine signalling
Cyclic guanosine monophosphate
Collagen Type II
Cyclic GMP
Confluency
Cell Differentiation
Natriuretic Peptide, C-Type
General Medicine
NPR1
Brain natriuretic peptide
Blotting, Northern
Molecular biology
NPR2
Rats
Blotting, Southern
chemistry
Guanylate Cyclase
Receptors, Parathyroid Hormone
Collagen Type X
Thymidine
Subjects
Details
- ISSN :
- 09148779
- Volume :
- 20
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of bone and mineral metabolism
- Accession number :
- edsair.doi.dedup.....b54ab94e0807cd0445703be27197b1ec