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Identification of strong promoters based on the transcriptome of Bacillus licheniformis
- Source :
- Biotechnology Letters. 39:873-881
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- To expand the repertoire of strong promoters for high level expression of proteins based on the transcriptome of Bacillus licheniformis. The transcriptome of B. licheniformis ATCC14580 grown to the early stationary phase was analyzed and the top 10 highly expressed genes/operons out of the 3959 genes and 1249 operons identified were chosen for study promoter activity. Using beta-galactosidase gene as a reporter, the candidate promoter pBL9 exhibited the strongest activity which was comparable to that of the widely used strong promoter p43. Furthermore, the pro-transglutaminase from Streptomyces mobaraensis (pro-MTG) was expressed under the control of promoter pBL9 and the activity of pro-MTG reached 82 U/ml after 36 h, which is 23% higher than that of promoter p43 (66.8 U/ml). In our analyses of the transcriptome of B. licheniformis, we have identified a strong promoter pBL9, which could be adapted for high level expression of proteins in the host Bacillus subtilis.
- Subjects :
- 0301 basic medicine
Operon
030106 microbiology
Bioengineering
Bacillus subtilis
Applied Microbiology and Biotechnology
Transcriptome
03 medical and health sciences
Promoter activity
Bacillus licheniformis
RNA, Messenger
Promoter Regions, Genetic
Gene
Genetics
biology
Sequence Analysis, RNA
Promoter
Gene Expression Regulation, Bacterial
General Medicine
biology.organism_classification
Molecular biology
RNA, Bacterial
030104 developmental biology
Genes, Bacterial
Stationary phase
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....b53aac6ea70c3a30cd350b3fc27310a7