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Overexpression of GhMPK3 from Cotton Enhances Cold, Drought, and Salt Stress in Arabidopsis

Authors :
Hengling Wei
Salisu Bello Sadau
Sani Muhammad Tajo
Bello Babatunde Kazeem
Shuxun Yu
Sani Ibrahim
Adeel Ahmad
Source :
Agronomy, Vol 11, Iss 1049, p 1049 (2021), Agronomy, Volume 11, Issue 6
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Cotton production is hampered by a variety of abiotic stresses that wreak havoc on the growth and development of plants, resulting in significant financial losses. According to reports, cotton production areas have declined around the world as a result of the ongoing stress. Therefore, plant breeding programs are concentrating on abiotic stress-tolerant cotton varieties. Mitogen-activated protein kinase (MAPK) cascades are involved in plant growth, stress responses, and the hormonal signaling pathway. In this research, three abiotic stresses (cold, drought, and salt) were analyzed on GhMPK3 transformed Arabidopsis plants. The transgenic plant’s gene expression and morphologic analysis were studied under cold, drought, and salt stress. Physiological parameters such as relative leaf water content, excised leaf water loss, chlorophyll content, and ion leakage showed that overexpressed plants possess more stable content under stress conditions compared with the WT plants. Furthermore, GhMPK3 overexpressed plants had greater antioxidant activities and weaker oxidant activities. Silencing GhMPK3 in cotton inhibited its tolerance to drought stress. Our research findings strongly suggest that GhMPK3 can be regarded as an essential gene for abiotic stress tolerance in cotton plants.

Details

ISSN :
20734395
Volume :
11
Database :
OpenAIRE
Journal :
Agronomy
Accession number :
edsair.doi.dedup.....b528eb6bcb61af26e563651bdeb59317
Full Text :
https://doi.org/10.3390/agronomy11061049