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PTPN1 promotes the progression of glioma by activating the MAPK/ERK and PI3K/AKT pathways and is associated with poor patient survival
- Source :
- Oncology Reports.
- Publication Year :
- 2019
- Publisher :
- Spandidos Publications, 2019.
-
Abstract
- Glioma is the most common primary brain tumor and is characterized by a poor prognosis. Protein tyrosine phosphatase 1B (PTPN1), as a non‑transmembrane protein tyrosine phosphatase, has been reported to serve a critical role in different diseases, including cancer. However, the role of PTPN1 in the progression of glioma remains unclear. The present study investigated the expression and clinicopathological characteristics of PTPN1 by analyzing the data from The Cancer Genome Atlas and 136 patients with glioma. It was indicated that PTPN1 was overexpressed in glioma tissues and served as a predictor for poor prognosis in patients with glioma. In addition, a series of in vitro experiments were performed to examine the underlying mechanism of PTPN1 overexpression and the clinical prognosis in patients with glioma. Knockdown of PTPN1 by small interfering RNA suppressed proliferation of glioma cells, including SF295 and A172. In addition, cell mobility was also inhibited by PTPN1 knockdown, downregulating the expression of matrix metallopeptidase 2 (MMP‑2) and MMP‑9. As indicated by western blot analysis, the mitogen‑activated protein kinase (MAPK)/extracellular‑signal‑regulated kinase (ERK) signaling pathway and the phosphatidylinositol 3‑kinase (PI3K)/AKT serine/threonine kinase (AKT) signaling pathway was regulated by PTPN1, while knockdown of PTPN1 significantly suppressed the MAPK/ERK and PI3K/AKT pathways, in addition to the downstream oncogenic transcription factor MYC Proto‑Oncogene. In conclusion, it was demonstrated that PTPN1 is upregulated in glioma tissue and the overexpression of PTPN1 predicted the poor prognosis of patients with glioma. PTPN1 promotes the progression of glioma by activating the MAPK/ERK and PI3K/AKT pathways.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
Cancer Research
MAP Kinase Signaling System
Apoptosis
Protein tyrosine phosphatase
Biology
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Cell Movement
Glioma
Biomarkers, Tumor
Tumor Cells, Cultured
medicine
Humans
Extracellular Signal-Regulated MAP Kinases
Protein kinase B
PI3K/AKT/mTOR pathway
Cell Proliferation
Protein Tyrosine Phosphatase, Non-Receptor Type 1
Oncogene
Brain Neoplasms
Kinase
General Medicine
Middle Aged
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
Survival Rate
030104 developmental biology
Oncology
Case-Control Studies
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Female
PTPN1
Proto-Oncogene Proteins c-akt
Follow-Up Studies
Signal Transduction
Subjects
Details
- ISSN :
- 17912431 and 1021335X
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....b524ce119ae08cb5e975e8e26b6333cc