Back to Search
Start Over
A 'turn-off' red-emitting fluorophore for nanomolar detection of heparin
- Source :
- Physical chemistry chemical physics : PCCP. 20(19)
- Publication Year :
- 2018
-
Abstract
- A simple fluorophore bearing a diethylaminocoumarin donor and a pyridinium acceptor was synthesized and utilized for the ultra-sensitive detection of heparin. The synthesized dicationic push-pull coumarin derivative emits strongly in the red-region (665 nm) and detects nanomolar concentrations (14.8 nM to 148 nM) of heparin in HEPES buffer and FBS serum solutions. The dication exhibits excellent fluorescence selectivity and sensitivity towards heparin over its analogues such as chondroitin 4-sulfate (CS), hyaluronic acid (HA) and dextran. This fluorescence assay is a convenient, sensitive method for monitoring heparin levels in biological samples. These findings were confirmed using coarse-grained Monte Carlo simulations, which provide us with a rationale for the selective binding of heparin.
- Subjects :
- Fluorophore
Magnetic Resonance Spectroscopy
General Physics and Astronomy
02 engineering and technology
Biosensing Techniques
010402 general chemistry
01 natural sciences
Sensitivity and Specificity
chemistry.chemical_compound
Limit of Detection
medicine
Computer Simulation
Physical and Theoretical Chemistry
Hyaluronic Acid
Fluorescent Dyes
Detection limit
Binding Sites
Chemistry
Heparin
Chondroitin Sulfates
Dextrans
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
Dication
Dextran
Spectrometry, Fluorescence
Pyridinium
0210 nano-technology
Selectivity
HEPES
Nuclear chemistry
medicine.drug
Protein Binding
Subjects
Details
- ISSN :
- 14639084
- Volume :
- 20
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- Physical chemistry chemical physics : PCCP
- Accession number :
- edsair.doi.dedup.....b51f6feb3e5629a7aee3247f4c15cfcb